Anti-miR-155反义寡核苷酸对A549细胞Wee1表达的影响  

Effect of anti-miR-155 oligonucleotides on Wee1 expression in A549 cells

作　　者：曹学武[1] 张海萍[2] 陈正堂[3] 

机构地区：[1]成都军区昆明总医院肿瘤科,云南昆明650032 [2]成都军区昆明总医院心血管内科,云南昆明650032 [3]第三军医大学新桥医院全军肿瘤研究所,重庆400037

出　　处：《西部医学》2013年第9期1298-1300,1303,共4页Medical Journal of West China

基　　金：国家自然科学基金资助项目(30672076);国家重点基础研究发展"863"计划资助项目(2007AA02Z129)

摘　　要：目的观察anti-miR-155反义寡核苷酸(AMOs)对A549细胞Wee1表达的影响。方法采用特异性anti-miR-155反义寡核苷酸抑制A549细胞内成熟miR-155的活性,realtime quantitive RT-PCR测定Wee1mRNA表达量,Western blot测定Wee1蛋白和Phospho-cdc2(Tyr15)蛋白的表达量。结果 100nmol/L浓度的AMOs处理的A549细胞中Wee1mRNA表达量与对照组A549细胞中Wee1mRNA表达量相比无显著性差异(P>0.05);与对照组相比,100nmol/L浓度的AMOs处理的A549细胞Wee1蛋白和Phospho-cdc2(Tyr15)蛋白的表达量显著增加(P<0.05)。结论采用AMOs抑制A549细胞内高水平表达miR-155活性后,可显著增强Wee1蛋白的表达。Objective To investigate the effect of anti-miR-155 oligonucleotides on Wee1 expression in A549 cells. Methods Anti-miR-155 oligonucleotides （AMOs） were used to inhibit the activity of mature miR-155 in A549 cells. Ex- pression level of Weel mRNA was measured by realtime quantitive RT-PCR. Expression levels of Weel protein and Phospho-cdc2 （Tyrl5） protein were measured by Western blot. Results AMOs at the concentration of 100nmol/L did not exert significant effect on expression level of Wee1 mRNA. Compared with control group, Weel expression and Phospho-cdc2 （Tyr15） expression in A549 cells treated with 100nmol/L AMOs was significantly increased. Conclusion After inhibition of activity of mature miR-155 by AMOs, expression of Weel protein can be significantly enhanced.

关 键 词：A549 MIR-155 反义寡核苷酸 Wee1 表达 

分 类 号：Q813[生物学—生物工程]