Purification and Characterization of a Novel Tetradecapeptide from Ginseng Polypeptides with Enhancing Memory Activity for Mice  

作　　者：LUO Hao-ming JIANG Rui-zhi YANG Xiao-hong CHEN Ying-hong HONG Tie WANG Ying 

机构地区：[1]School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P R. China [2]Jilin Academy of Chinese Medicine and Material Medical Science, Changchun 130012, P. R. China

出　　处：《Chemical Research in Chinese Universities》2013年第4期638-641,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（No.81173532）, the Science and Technology Development Project of Jilin Province, China（No.201201086）, the Traditional Chinese Medicine Science and Technology Project from Jilin Provincial Administration of Traditional Chinese Medicine, China（No.2012-114）.

摘　　要：Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaecharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradeeapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-eleetrospray ionization-quadrupole-time of flight-mass spectrometry（LC-ESI-Q-TOF-MS） with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.Aiming at isolating and investigating the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory activity, the authors characterized one ingredient. To separate the oligosaecharides and polypeptides, a DEAE-Sephadex A-50 colum was used. The enhanced memory activity in mice was studied by Mirros water maze tesk in mice. The dose of oligosacchrides, polypeptides or Piracetam was 30 mg/kg per day with intraperitoneal administration. The oligosaccharides did not show enhancing memory effect, but polypeptides did show. This result demonstrates that the active ingredients of the aqueous extract from Panax ginseng which showed enhancing memory effect was polypeptides. The purification of the polypeptides was performed on a Sephadex G-25 column. A novel tetradeeapeptide was purified from the polypeptides and its structure was determined by liquid chromatography-eleetrospray ionization-quadrupole-time of flight-mass spectrometry（LC-ESI-Q-TOF-MS） with the amino acid sequence of Lys-Ser-Leu-Thr-Leu-Thr-Ser-Ser-Leu-Ser-Tyr-Thr-Asp-Ser.

关 键 词：Panax ginseng POLYPEPTIDE Tetradecapeptide Enhanced memory activity Liquid chromatography-tandemmass spectrometry（LC-MS/MS） 

分 类 号：Q983.5[生物学—人类学] Q516