氨转运蛋白Rhbg和Rhcg在低蛋白饮食大鼠肾脏的表达  

Expression of renal ammonia transporters Rhbg and Rhcg in rats fed with low-protein diet

作　　者：李树平[1] 罗静[2] 

机构地区：[1]大同煤矿集团有限责任公司总医院检验科,037003 [2]山西医科大学第二医院风湿科

出　　处：《中国药物与临床》2013年第9期1144-1146,共3页Chinese Remedies & Clinics

基　　金：山西省出国留学基金(2008102);山西省科学技术厅中美合作项目(2011081071)

摘　　要：目的观察氨转运蛋白Rhbg、Rhcg在低蛋白饮食大鼠肾脏集合管的表达。方法利用蛋白印迹法和实时聚合酶链反应(PCR)分别检测低蛋白饮食组和正常对照组氨转运蛋白Rhbg和Rhcg在大鼠肾脏皮质、内髓、外髓的表达。结果低蛋白饮食可以明显增加Rhbg在大鼠肾脏皮质的表达(P<0.05),但不改变Rhcg在大鼠肾脏皮质、内髓和外髓的表达。结论低蛋白饮食可能会改变氨转运蛋白在大鼠肾脏的表达。Objective To determine the effect of ammonia transporters Rhbg and Rhcg in renal collecting ducts in rats fed with low-protein diet. Methods The expression of Rhbg and Rhcg in renal cortex, outer medulla and inner medulla was detected by using Western blotting and real-time polymerase chain reaction in rats fed with low-protein diet and healthy rats. Results Rats fed with low-protein diet had significantly augmented Rhbg expres- sion in the cortex （P〈0.05） yet not altered Rhcg expression in the cortex and outer and medulla. Conclusion Low- protein diet results in altered renal ammonia transporter expression in rats.

关 键 词：载体蛋白质类 大鼠 Sprague—Dawley 膳食 限制蛋白质 

分 类 号：R341[医药卫生—基础医学]