压力对培养人眼小梁细胞水通道蛋白1mRNA表达及蛋白质合成的影响  

Effect of Pressure on mRNA Expression and Protein Synthesis of Aquaporin-1 in Cultured Human Trabecular Meshwork Cells

作　　者：彭洁[1] 樊映川[1] 张虹[2] 

机构地区：[1]四川省医学科学院,四川省人民医院眼科,成都610072 [2]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《华中科技大学学报（医学版）》2013年第4期442-445,450,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：四川省卫生厅资助项目(No.100539)

摘　　要：目的研究人眼小梁细胞水通道蛋白1(AQP1)在不同压力下mRNA表达及蛋白质合成的变化,探讨AQP1在青光眼发病中的作用。方法培养人眼小梁细胞,分别给予20、40、60及80mmHg(1mmHg=0.133kPa)的压力,以不加压组为对照组,用RT-PCR和Western blot对小梁细胞AQP1mRNA和蛋白质含量变化进行定性及定量观察。结果压力持续48h后,人眼小梁细胞中AQP1mRNA及蛋白质含量随压力增高先增高后下降。压力20mmHg与40mmHg组AQP1mRNA及蛋白质含量较对照组逐渐增多,压力60mmHg和80mmHg组AQP1mRNA及蛋白质含量较对照组逐渐减少,各组AQP1mRNA及蛋白质与对照组比较差异均有统计学意义(F=251.95,P<0.01;F=447.23,P<0.01)。组间两两比较差异均有统计学意义(均P<0.01)。结论压力改变培养人眼小梁细胞AQP1mRNA表达及蛋白质合成,可能成为导致或加重青光眼的因素之一。Objective To investigate the effect of different pressures on AQP1mRNA expression and protein synthesis in human trabecular meshwork cells in an attempt to elucidate the role of AQP1in the development of glaucoma.Methods Human trabecular meshwork cells were cultured and subjected to different pressures（20,40,60and 80mmHg）.No pressure group served as control.The mRNA and protein expression of AQP1in trabecular meshwork cells under different pressures was determined qualitatively and quantitatively by RT-PCR and Western blot respectively.Results Within 48h,the expression levels of AQP1mRNA and protein were initially increased and then decreased with pressure increasing.AQP1mRNA and protein expression levels were significantly enhanced in 20mmHg and 40mmHg groups relative to the control group and they were shown to markedly decrease in 60mmHg and 80mmHg groups when compared with the control group.There were statistically significant differences in the expression of AQP1mRNA and protein between each pressure group and control group（F=251.95,P 0.01,F=447.23,P〈0.01）.There existed statistically significant difference in the expression of AQP1mRNA and protein between each two groups（P〈0.01）.Conclusion Pressure may induce or aggravate glaucoma by changing the expressions of AQP1mRNA and protein.

关 键 词：小梁细胞 水通道蛋白1 压力 青光眼 

分 类 号：R775[医药卫生—眼科]