川芎嗪对人肺腺癌细胞株AGZY的增殖抑制及逆转耐药机制的研究  

Studies of TMP on restraining proliferation and reversing multidrug resistance in human lung adenocar- cinoma cell line

作　　者：刘静蕾[1] 姜季[2] 张明岩[3] 王影[3] 刘永蕊[1] 王艳[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院肿瘤内科,哈尔滨150081 [2]哈尔滨医科大学附属肿瘤医院同位素科 [3]哈尔滨医科大学附属肿瘤医院检验科

出　　处：《实用肿瘤学杂志》2013年第4期303-307,共5页Practical Oncology Journal

基　　金：黑龙江省教育厅科研项目资助（12521283）

摘　　要：目的探讨川芎嗪（Tetramethylpyrazine，TMP）对人肺腺癌细胞株AGZY抑制增殖、逆转耐药的作用。方法应用MTT法检测TMP对耐阿霉素的AGZY细胞耐药情况的影响；通过绘制生长曲线、细胞集落形成实验观察TMP对AGZY细胞增殖情况的影响；应用穿膜实验检测TMP对AGZY细胞侵袭、转移情况的影响；应用Westernblot方法检测TMP对AGZY细胞nm23、MDRI蛋白表达情况的影响。结果TMP作用后，AGZY细胞对阿霉索的耐药性下降，UC50降低（P〈0．05）；细胞的生长、增殖能力下降；细胞的穿膜能力降低（P〈0．05）；TMP作用后，AGZY细胞中rim23蛋白表达上调、MDR1蛋白表达下调。结论TMP可抑制AGZY细胞的增殖，细胞的侵袭和转移能力的抑制与nm23表达的上调有关，通过下调MDR1的表达达到逆转细胞耐药的作用。。Objective To study the effects of tetramethylphylpyrazine（TMP） on cell proliferation and re- versing drug resistance in human lung adenocareinoma cell llne AGZY. Methods The drug sensitivity of AGZY cells to ADM was measured by MTT assay. The cell proliferation states were investigate by cell growth curve and cell colony formation test. The migration and invasion of AGZY cells were measured with cell invasion assay. The protein expression levels of nm23 and MDR1 were determined by western blot analyses. Results The IC5o and drug - resistant index of ADM were decreased by TMP, Cell proliferation ability was decreased obviously. Cell in- vasion ability was decreased. The expression of rim23 was upregulated, and the expression of MDRI was downreg- ulated. Conclusion ＇IMP could inhibit the growth of AGZY cell. TMP could enhance the AGZY cell chemosensi- tivity to ADM,and it maybe related with the down -regulation of MDR1. TMP can restrain AGZY cell invasion and metastasis,and it may have relationship with the upregulation of nm23.

关 键 词：川芎嗪 人肺腺癌 转移 多药耐药 

分 类 号：R734.2[医药卫生—肿瘤]