羊布鲁菌外膜蛋白Bp-26基因的克隆及其原核表达  

Cloning and Prokaryotic Expression of OMP Bp-26 Gene of Brucella melitensis

作　　者：史巧芸[1] 荣辉[1] 郭莳雨[1] 贾晓晓[1] 张珈宁[1] 朱华培[1] 杜丽[1] 成鹰[1] 焦寒伟[1] 王凤阳[1] 

机构地区：[1]海南大学农学院,海南省热带动物繁育与疫病研究重点实验室,海口市动物基因工程重点实验室,海南海口570228

出　　处：《动物医学进展》2013年第9期15-17,共3页Progress In Veterinary Medicine

基　　金："863"计划项目(2011AA100302);"863"计划项目(2013AA102524)

摘　　要：应用PCR扩增羊布鲁菌M5-90株基因组DNA,得到大小为753bp的Bp-26基因,将其克隆入pMD20-T载体上,测序正确后,构建重组质粒pET-28a-Bp-26,转化到大肠埃希菌BL21(DE3)中,经IPTG诱导其表达,用Western blot鉴定蛋白。结果表明,成功构建了pET-28a-Bp-26原核表达载体,并在E.coli BL21中表达Bp-26基因,为开展羊布鲁菌Bp-26目的蛋白的抗原性分析和功能研究奠定了基础。Bp-26 gene was amplified at lengths of 753 bp by PCR from genomic DNA of Brucella melitensis, and was ligated into pMD20-T. The constructed recombinant plasmid pET-28a-Bp-26 was transformed into E. coli BL21 （DE3） for expression under induction of IPTG. The products were identified by SDS-PAGE and Western blot successfully, which provides important information for further function analysis and anti- genic analysis of Bp-26 protein.

关 键 词：羊布鲁菌 Bp-26基因 克隆 原核表达 

分 类 号：Q786[生物学—分子生物学]