青海省海晏县犬粪样中贾第鞭毛虫的检测  

Detection of Giardiain Dog Feces in Haiyan County of Qinghai Province

作　　者：王光华[1] 蔡其刚[1] 王戈平[1] 陆艳[1] 李秀萍[1] 叶成玉[1] 牛小迎[1] 马利青[1] Panagiotis Karanis 

机构地区：[1]青海省畜牧兽医科学院,青海西宁810016 [2]Medical and Molecular Parasitology Laboratory, University of Cologne, Germany

出　　处：《动物医学进展》2013年第9期29-32,共4页Progress In Veterinary Medicine

基　　金：农业部948专项经费资助(2009-Z-48)

摘　　要：从青海省海晏县采集10只牧羊犬粪样用于贾第鞭毛虫检测。所有样品经饱和盐水浓缩法和蔗糖密度梯度离心等方法处理后采用光学显微镜、免疫荧光试验(IF)和套式PCR方法进行检测。结果显示,其中1个样品为阳性(10%),PCR扩增片段长度为274bp,命名为QHGC201101株。测序结果与肠贾第鞭毛虫3VR株(HQ616609)核糖体小亚基RNA的同源性为100%,表明分离的虫种为肠贾第鞭毛虫,属于人兽共患型病原。10 dog feces were collected from Haiyan county of Qingh Samples were examined by microscope, IFT and PCR methods aft ai province for the detection of Giardia. er saturated salt solution floatation and discontinuous sucrose density gradient centrifugation. The results showed that one sample was positive with Giardia and the positive rate was 10~. A fragment of 274 bp was amplified specifically. As demon strated by 18 S rRNA gene analysis, the homology of QHGC201101 strain with HQ616609 was 100%. The isolated species was Giardia intestinalis, and it is a zoonosis pathogen.

关 键 词：犬 贾第鞭毛虫 18 S RRNA 套式PCR 

分 类 号：S852.722[农业科学—基础兽医学]