细胞块技术联合免疫细胞化学检测浆膜腔积液中腺癌细胞的价值  

The clinical value of the cell blocks method and immunochemistry on adenocarcinoma cell in serous cavity effusions

作　　者：孙亚军[1] 李梅[1] 吴建农[1] 付先利[1] 肖栩[1] 刘颖[1] 

机构地区：[1]江苏大学附属医院,212001

出　　处：《实用医技杂志》2013年第9期933-935,共3页Journal of Practical Medical Techniques

基　　金：江苏大学医学临床科技发展基金(JLY20120168)

摘　　要：目的探讨浆膜腔积液中腺癌细胞细胞块技术联合免疫细胞化学检测的临床价值。方法选用浆膜腔积液转移性腺癌共60例,每例均制备常规细胞学涂片和细胞块,并用细胞块切片做CK7抗体、CK18抗体、癌胚抗原(CEA)、甲状腺转录因子(TTF)-1、Vimentin、Calretinin、间皮细胞抗原(MC)行免疫细胞化学染色。结果 60例浆膜腔积液标本中CK7抗体、CK18抗体、CEA对于转移性腺癌的敏感性分别为100%(60/60)、95%(57/60)和75%(45/60),特异性分别为100%(60/60)、87%(52/60)和80%(48/60)。结论浆膜腔积液细胞学中细胞块技术联合免疫细胞化学检测与传统细胞学涂片相比,明显提高了细胞学病理诊断的敏感性和准确性,使细胞学诊断上升到免疫学水平,是一种值得推广的方法。Objective To explore the clinical value of cell block and immunochemistry on adenocarcinoma cell in serous cavity effusions. Methods Sixty cases of metastatic adenocarcinoma With serous cavity effusions Were choosed and prepared routing cell smears and cell blocks for each case. Cell blocks sections would be applied to the detection of immunocytochemical staining for the cell block sections for CKT, CK18, CEA, TrF-1, Vimentin, Calretinin and MC. Results In60 of serous cavity effusion samples, CK7, CKI8, CEA sensitivity to metastatic adenocarcinoma were 100% （60160）, 95%（57/60） and 75%（45/60）： The specificity were 100%（60/60）, 87%（52/60） and 80%（48/60）. Conclusion Compared with the conventional cytology smear in cavity effusion cytology, Cell block technique and immunocytochemical detection could improve the sensitivity and accuracy of cytological diagnosis significantly and rises to immunological level. It is a approach that is worthy spreading,

关 键 词：腺癌 免疫组织化学 组织细胞学制备技术 

分 类 号：R730.4[医药卫生—肿瘤]