人胚胎视网膜神经上皮移植于兔视网膜的初步实验研究  

Human embryonic neural retina transplantation to rabbit retina

作　　者：赵春阳[1] 

机构地区：[1]广东省江门市人民医院眼科,529000

出　　处：《眼科新进展》2000年第5期319-321,共3页Recent Advances in Ophthalmology

基　　金：广东省医药卫生青年科学研究基金资助

摘　　要：目的　探讨人胚胎视网膜神经上皮移植于兔视网膜的方法和效果。方法　将人胚胎 (2 .5～ 3.5 mo)片状视网膜神经上皮移植于新西兰白兔视网膜脉络膜缺损区 (后段外路移植法 ) ,共 6例 ,2 0 g· L- 1 环胞素 A及 1g· L- 1 地塞米松滴眼 ,术后 3mo电镜观察。结果　术后 3m o植片成活 5例 ,可见视锥、视杆细胞 ,较少的神经节细胞和较多的无长突细胞、米勒细胞生长。未发现参与排斥反应的细胞 ,但感光细胞发育欠成熟 ,细胞间突触联结不明显 ,未见内界膜和神经纤维层。结论　人胚胎视网膜神经上皮实验性移植成活率较高 ,神经上皮分化但细胞发育欠成熟 ;2 0 g· L- 1 环胞素 A及 1g· L- 1Objective To evaluate the results of the experimental transplantation of human embryonic neural retina to rabbits.Methods Neural retina from human embryo of 2 5～3.5 months were transplanted to the site where choroids and retina were excised through posterior sclera in 6 adult rabbits. 20g·L -1 Cyclosporine oil and 1g·L -1 Dexamethasone eyedrops were locally used to control the rejection 3 times a day postoperatively. The transplants were observed under electron microscope 3 months later.Results In 5 survival grafts out of 6 cases, the cone and rod cells,a few ganglion cells and relatively a lot of amacrine cells and Muller cells were found under electron microscope. But the photoreceptors and ganglion cells were immature in development,the synaptic connection was not evident, the inner limiting membrane and nerve fiber layer were not found. The immune cells involved in rejection were not observed.Conclusions The survival rate of the transplants of the human embryonic neural retinas is relatively high, the differentiation of the grafts is confirmed under electron microscope, but the development of the neural cells is not good enough to maturity. The rejection is controlled in a good state with Cyclosporine and Dexamethasone locally administrated.

关 键 词：视网膜移植 胚胎 视网膜神经上皮 

分 类 号：R779.6[医药卫生—眼科]