高浓度葡萄糖对视网膜毛细血管周细胞的影响  

Damage of high glucose on retinal capillary pericytes

作　　者：程丽霞[1] 董砚虎[1] 王家富[1] 

机构地区：[1]山东省潍坊市人民医院内分泌科,261041

出　　处：《眼科新进展》2000年第5期339-341,共3页Recent Advances in Ophthalmology

基　　金：山东省教委资助

摘　　要：目的　观察高浓度葡萄糖对体外培养的视网膜毛细血管周细胞的影响 ,探讨糖尿病视网膜病变 (DR)的发病机制。方法　以新鲜牛眼球为材料 ,分离并培养周细胞 ;控制培养液中葡萄糖的浓度 ,采用形态学观察、乳酸脱氢酶 (L DH)的测定、3H- Td R掺入法及液体闪烁技术 ,研究高糖对周细胞的作用。结果　周细胞在高糖 (10、2 0、40 mmol· L- 1 )培养液及不同培养时间 (4 8、96 h)下 ,L DH水平与对照组 (生理浓度葡萄糖 ,5 mm ol· L- 1 )比较无显著性差异 (P>0 .0 5 )。3H- Td R掺入法研究结果显示 ,高糖 (分别培养 48、96、144 h)对周细胞的增殖有明显抑制作用 ,随时间延长和葡萄糖浓度升高 ,抑制率增长 ,其中 40 m mol· L- 1葡萄糖培养 144 h对周细胞的抑制率达 78.0 9% .结论　高浓度葡萄糖对体外培养周细胞的增殖有明显抑制作用 ,并随葡萄糖浓度升高和时间延长 。Objective To observe the effect of high glucose on retinal capillary pericytes in vitro and research the pathogenesis of diabetic retinopathy (DR). Methods Retinas dissected from eyes of fresh killed cattle were separated and retinal capillary pericytes were cultured. The effect of high glucose on pericytes was evaluated by measurement of lactate dehydrogenase(LDH), 3H thymidine incorporation and liquid scintillation techniques.Morphological changes were observed under microscope and electric microscope.Results There was no significant difference in LDH activity in the supernatants between high glucose(10、20、40mmol·L -1 ) and normal glucose(5mmol·L -1 )cultured for 48h or 96h. High glucose had an effective inhibition on pericytes' proliferation cultured for 48h,96h and 144h, and the inhibition rate increased when the glucose concentration rised and time passed by. The inhibition rate of pericytes was 78.09% after cultured in high glucose (40mmol·L -1 ) for 144h. Conclusion High glucose has an effect inhibition on pericytes' proliferation, and the inhibition rate increase when the glucose concentration rises and time passes by.

关 键 词：视网膜 毛细血管周细胞 葡萄糖 细胞增殖 

分 类 号：R744.1[医药卫生—神经病学与精神病学] R587.2[医药卫生—临床医学]