miRNA-106a表达水平对食管鳞癌患者预后的影响  

Effect of miRNA-106a expression on the prognosis of patients with esophageal squamous cell carcinoma

作　　者：袁嘉敏[1] 毛伟敏[1] 罗君[1] 彭兵锋[1] 郑智国[1] 凌志强[1] 

机构地区：[1]浙江省肿瘤医院浙江省肿瘤研究所浙江省胸部肿瘤肺、食管诊治技术研究重点实验室,杭州310022

出　　处：《中华肿瘤杂志》2013年第8期590-594,共5页Chinese Journal of Oncology

基　　金：浙江省自然科学基金（Y2080749、Y2091110）;浙江省人事部优秀留学回国人员科技活动项目择优基金（2008A004）;浙江省科技计划基金（No.2009C33143）;教育部优秀留学回国人员科研基金（2010609）;卫生部科研基金（WKJ2010-2-004、WKJ2011-2-015）;浙江省医药卫生平台重点资助计划（2011ZDA004）;浙江省重大科技专项计划项目（2011C13039-1）

摘　　要：目的探讨miRNA-106a基因表达水平与食管鳞癌患者临床病理特征和预后的关系。方法应用实时荧光定量PCR方法检测81例食管鳞癌组织和相应正常组织中miRNA-106a基因的表达量，采用免疫组化法检测p53、人表皮生长因子受体2（HER-2）、DNA拓扑异构酶Ⅱ（TopoⅡ）和多药耐药相关蛋白（MRP）的表达情况，分析miRNA-106a表达与食管鳞癌患者临床病理特征、相关蛋白表达和预后的关系。结果81例食管鳞癌组织中，miRNA-106a基因低表达48例（59．3％），高表达11例（13．6％），正常表达22例（27．2％）。miRNA．106a基因表达水平与食管鳞癌患者的淋巴结转移、病理分期和神经浸润显著相关（均P〈0．05）。miRNA-106a基因表达水平与p53蛋白表达显著相关（P=0．006），与HER-2、Topo11和MRP蛋白表达无关（均P〉0．05）。miRNA-106a基因表达水平与食管鳞癌患者的总生存时间（OS）无关（P=0．486），与无进展生存时间（PFS）有关（P=0．032）。多因素Cox回归分析显示，食管鳞癌患者的PFS与患者年龄、性别、肿瘤长度、T分期、分化程度、神经浸润和miRNA．106a表达水平无关（均P〉0．05），而与淋巴结转移有关（P=0．029）。结论miRNA-106a基因在食管鳞癌组织中低表达，具有抑癌基因的功能，可作为生物标记评估食管鳞癌患者的预后。Objective To study the expression of miRNA-106a gene in esophageal squamous cell carcinoma （ESCC） and its association with clinicopathological features and prognosis of ESCC patients. Methods Real-time fluorescence quantitative polymerase chain reaction （PCR） assay was used to determine the expression of miRNA-106a gene in esophageal cancer tissue and corresponding normal mucosa of 81 cases. Immunohistochemical technique was applied to detect the expression of p53, human epidermal growth factor receptor 2 （ HER-2）, DNA topoisomerase II （ Topo Ⅱ ） and multidrug resistance-associated protein （MRP）. The association of miRNA-106a expression with clinieopathological features, expression of related proteins, and prognosis of the patients was analyzed. Results Among the 81 eases, under-expression of miRNA-106a gene was found in 48 cases （59.3%）, normal expression in 22 cases （27.2%）, and overexpression in 11 cases （13.6%）. The expression of miRNA-106 gene was significantly associated with lymph node metastasis, pathological stage, and nerve invasion （ all P 〈 0.05 ） , significantly associated with expression of p53 （P = 0.006）, and not significantly associated with expressions of HER-2, Topo 11 and MRP proteins （ all P 〉 0.05 ）. The expression of miRNA-106a gene was also significantly associated with progression-free survival （ PFS, P = 0. 032 ）, but not significantly with overall survival （ OS, P = 0. 486 ）. The results of Cox multivariate regression analysis showed that the PFS of ESCC patients was significantly correlated with lymph node metastasis （ P =0. 029 ）, but not correlated with the age, gender, tumor length, T stage, degree of differentiation, nerve invasion, and miRNA-106a expression （all P 〉 0.05 ）. Conclusions In esophageal squamous cell carcinomas, the miRNA-106a gene is under-expressed, with tumor suppressor function, and may be regarded as a biological marker to assess the prognosis in patientswith esophageal squamous cell carcinoma.

关 键 词：食管肿瘤 miRNA-106a基因 基因表达 预后 

分 类 号：R735.1[医药卫生—肿瘤]