人肝星形细胞胰高血糖素样肽1受体的表达  

The expression of glucagon-like peptide 1 receptor in human hepatic stellate cell

作　　者：吴凌康[1] 厉有名[2] 刘英超[1] 施维群[1] 吴峰[2] 茹清静[1] 

机构地区：[1]浙江中医药大学附属第二医院肝病科,杭州310005 [2]浙江大学医学院附属第一医院消化科,杭州310009

出　　处：《国际流行病学传染病学杂志》2013年第4期236-238,F0003,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省医药卫生科技计划（2013KYA144）

摘　　要：目的证实胰高血糖素样肽1（GLP_1）受体在人肝星形细胞上的表达。方法体外培养人肝星形细胞并进行形态学鉴定。提取肝星形细胞mRNA并逆转录获得eDNA，RT-PCR法测定GLP-1受体mR．NA的表达，免疫印迹法测定GLP-1受体蛋白的表达情况。结果显微镜下可以观察到活化的肝星形细胞为扁平状，胞体大，具有发育良好的应力纤维，胞浆内缺乏脂肪滴。GLP-1受体eDNA片段长度为296bp，与GenBank公布的人GLP-1受体eDNA序列（NM．002062．3）相符度为100％。免疫印迹法表明人肝星形细胞样本GLP-1受体蛋白表达阳性。结论人肝星形细胞上存在GLP-1受体的表达。Objective To confirm the expression of glucagon-like peptide 1（GLP-1） receptor in human hepatic stellate cell （HSC）. Methods Human HSC was cultured in vitro and morphologically identified. The mRNA of HSC was extracted and reversely transcribed to obtain cDNA. The mRNA of GLP-1 receptor was determined by RT-PCR. The GLP- 1 receptor protein was determined by western blotting. Results The activating HSC were flat, with large cell body and well-developed stress fibers and without fat droplets in the cytoplasm under the microscope. The length of GLP-1 receptor cDNA fragment was 296 bp, which was 100% consistent with the human GLP-1 receptor cDNA sequence （NM- 002062.3） published in Gen Bank. The results of western blotting showed that there was GLP- 1 receptor protein expressed in human HSC. Conclusions The GLP-1 receptor express in human HSC.

关 键 词：肝星形细胞 GLP-1受体 免疫印迹 RT-PCR 抗肝纤维化 

分 类 号：R363[医药卫生—病理学]