全反式维甲酸对人食管鳞癌EC1细胞增殖、细胞周期和细胞凋亡的影响  

Effects of all-trans retinoic acid on proliferation,cell cycle and apoptosis of human esophageal squamous cancer EC1 cells

作　　者：姚义好[1,2] 丁艳霞[1,3] 张晋东[1,4] 王晓娜[1] 巫小龙[1] 黄幼田[1] 杨洪艳[1] 赵继敏[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理教研室,河南郑州450001 [2]华中科技大学同济医学院附属同济医院放射科,湖北武汉430030 [3]郑州大学第一附属医院内分泌科,河南郑州450052 [4]郑州大学第一附属医院心内科,河南郑州450052

出　　处：《肿瘤》2013年第8期690-694,共5页Tumor

基　　金：河南省教育厅自然科学研究计划项目(编号:2011A310013)

摘　　要：目的:探讨全反式维甲酸(all-tran retinoid acid,ATRA)对人食管鳞癌EC1细胞增殖、细胞周期和细胞凋亡的影响,并初步探讨其可能的机制。方法:分别以1、10和20μmol/L ATRA处理EC1细胞,应用MTT法检测细胞的增殖情况,FCM法检测细胞周期和细胞的早期凋亡,免疫细胞化学染色和蛋白质印迹法检测EC1细胞中p21和Bcl-2蛋白的表达。结果:与未经ATRA处理的对照组比较,1、10和20μmol/L ATRA可抑制EC1细胞的增殖,且呈剂量和时间依赖效应(P<0.05)。1、10和20μmol/L ATRA处理EC1细胞后,G0/G1期细胞比例[(66.58±0.30)%、(67.96±0.77)%和(75.11±2.24)%]高于对照组[(59.90±0.82)%](P<0.05),且细胞的早期凋亡率[(4.86±0.04)%、(7.35±0.04)%和(12.03±0.12)%]也高于对照组[(3.17±0.05)%](P<0.05)。ATRA作用后,EC1细胞中p21蛋白的表达水平上调,而Bcl-2蛋白的表达水平下调。结论:ATRA可以有效抑制食管鳞癌EC1细胞的增殖、阻滞细胞周期并诱导细胞凋亡,这个过程可能与p21表达上调和Bcl-2表达下调有关。Objective： To investigate the effects of ATRA （all-trans retinoic acid） on cell proliferation,cell cycle and apoptosis of human esophageal squamous cancer EC1 cells,and to explore its possible mechanism.Methods： The abilities of cellular proliferation of EC1 cells after treatment with 1,10 and 20 μmol/L ATRA were detected by MTT assay,and the cell cycle distribution and the apoptosis of EC1 cells were determined by flow cytometry.The expressions of p21 and Bcl-2 in EC1 cells after treatment with 1,10 and 20 μmol/L ATRA were detected by immunocytochemistry and Western blotting.Results： As compared with the EC1 cells without treatment （as a control）,the proliferation of EC1 cells was inhibited by ATRA in a dose- and time-dependent manner （P〈0.05）.The proportions of G 0 and G 1 cells were higher in EC1 cells after treatment with 1,10 and 20 μmol/L ATRA than that of the control cells （66.58± 0.30）%,（67.96±0.77）% and （75.11±2.24）% vs （59.90±0.82）%,P〈0.05.The apoptotic rates of EC1 cells treated with 1,10 and 20 μmol/L ATRA were higher than that of the control cells （4.86±0.04）%,（7.35± 0.04）% and （12.03±0.12）% vs （3.17±0.05）%,P〈0.05.The expression level of p21 in EC1 cells treated with ATRA was up-regulated,but the expression level of Bcl-2 was down-regulated.Conclusion： ATRAcan inhibit the proliferation of human esophageal squamous cancer EC1 cells and induce G 0 /G 1 phase blocking and cell apoptosis.This effect may be related with the up-regulation of p21 expression and down-regulation of Bcl-2 expression.

关 键 词：食管肿瘤 细胞增殖 细胞周期 细胞凋亡 全反式维甲酸 

分 类 号：R735.1[医药卫生—肿瘤]