尖端电喷雾萃取电离质谱技术诊断食管癌22例  

Tip-spray ionization mass spectrometry for diagnosis of esophageal cancer:An analysis of 22 cases

作　　者：赵宗盛[1] 徐建军[1] 陈立如[1] 温华[1] 陈焕文[2] 朱腾高[2] 魏益平[1] 

机构地区：[1]南昌大学第二附属医院,江西省南昌市330006 [2]东华理工大学江西省质谱科学与仪器重点实验室,江西省南昌市330013

出　　处：《世界华人消化杂志》2013年第23期2320-2324,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81160293;国家重大科学仪器设备开发专项基金资助项目;No.2011YQ170067-09~~

摘　　要：目的:探讨尖端电喷雾萃取电离质谱技术(tipspray ionization mass spectrometry,Tip-sprayMS)对食管癌(esophageal cancer,EC)诊断的应用.方法:收集22例EC及配对的癌旁组织,应用Tip-spray-MS技术对组织样品进行快速分析,调节Tip-spray离子源为正离子模式,质量范围50-1000 Da,喷雾电压为3.5 kV,喷雾距离为2 cm,透镜电压为65.0 V,离子传输管温度为150℃.运用Matlab软件主成分分析(principal component analysis,PCA)方法对所获质谱指纹谱进行统计学分析.结果:EC与癌旁组织的质谱指纹谱图中相对丰度有明显差异的质荷比(m/z)为104、154、175、611、655、699、743、787、831等处,其中m/z为611、655、699处丰度差异最显著,其所对应的物质为卟啉,运用PCA方法能够良好地区分EC及癌旁组织.结论:Tip-spray-MS联合PCA能有效鉴别EC及癌旁正常组织,有助于EC的早期、快速诊断.AIM： To discuss the application of tip-spray ion~ ization mass spectrometry （MS） in the diagnosis of esophageal cancer. METHODS： Tip-spray MS was used to analyze esophageal cancer tissue samples and matched tumor-adjacent esophageal tissue samples from 22 patients with esophageal cancer, Principal component analysis （PCA） was performed to process data from the MS fingerprint spectra RESULTS： Peptide peaks at m/z values of 104, 154, 175, 611, 655, 699, 743, 787 and 831 showed significantly different relative abundance. Peaks at m/z values of 611, 655 and 699, which cor- responded to porphyrin, had the most obvious difference. PCA could effectively distinguish esophageal cancer and tumor-adjacent normal tissue. CONCLUSION： Tip-spray MS combined with PCA can effectively distinguish esophageal cancer and tumor-adjacent normal tissue and therefore may contribute to the rapid diagnosis of esophageal cancer.

关 键 词：食管癌 快速诊断 尖端电喷雾萃取电离质谱 主成分分析 

分 类 号：R735.1[医药卫生—肿瘤]