流式细胞术在实验教学中的开发与应用  

Development and Application of Flow Cytometry in Experimental Teaching of Cell Biology

作　　者：张英[1] 黎宏宇[1] 李莉[1] 杨举[1] 成军[1] 郝林琳[1] 于浩[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《实验室研究与探索》2013年第7期206-208,216,共4页Research and Exploration In Laboratory

摘　　要：流式细胞术是一门新兴技术,广泛应用在细胞生物学相关教学及科研中,流式细胞术实验课通过流式细胞仪工作原理讲解、仪器演示和实验操作,帮助学生消化课堂的教学内容,以便了解、掌握这门新技术。笔者根据农业院校教学特点,开设了流式细胞术实验课,并设计了流式细胞仪的操作演示、EGFP和小鼠脾脏淋巴细胞亚类的检测3个教学内容。流式细胞术实验课教学结果表明,学生加深了对流式细胞仪基础知识的理解和掌握,细胞标本制备、上机检测和结果分析等,提升了学生的动手操作能力、科研分析及解决问题的能力。而且,提高了流式细胞仪的利用率,使学生掌握了流式细胞术和细胞的生物学特性,为学生在未来的科研实验中利用该项技术奠定了基础。Flow cytometry,an emerging technology,is widely used in teaching and research in cell biology.The subject of flow cytometry experiments includes explanation of principle of flow cytometry,instrument demonstrations and laboratory operations.The aim was to enable students to better digest the teaching content and master this new technology.Taking the characteristics of agricultural colleges into consideration,the flow cytometry experimental course was established.Practice shows that the experimental course enables students to better understand and master the basics of flow cytometry,cell sample preparation,hands-on testing and results analysis,which enhances students＇ hands-on skills,research analysis,and problem-solving ability.

关 键 词：流式细胞术 细胞生物学 实验教学 

分 类 号：Q291[生物学—细胞生物学]