细胞松弛素B对多头绒泡菌有丝分裂的影响  

Effects of Cytochalasin B upon Mitosis of Physarum polycephalum

作　　者：宋朝霞 邢苗[1] 曾宪录 

机构地区：[1]深圳大学生物工程系,深圳特区518060

出　　处：《Acta Genetica Sinica》2000年第1期83-89,共7页

基　　金：国家教委跨世纪人才基金

摘　　要：将细胞松弛素B(Cytochalasin B, CB)注入同步化的多头绒泡菌原质团,在光镜和电镜下跟踪观察其有丝分裂进程,发现多头绒泡菌进入有丝分裂的时间推迟,与未经CB处理的样品相比,在S期注入CB的样品进入有丝分裂的时间推迟35min;在G2早期注入CB的样品则推迟20min;在G2中期注入的推迟45min;在G2晚期注入的推迟60min,说明抑制肌动蛋白的功能则使有丝分裂受到明显影响。CB处理样品进入有丝分裂后的整个时间进程和核结构的动态变化与未经CB处理的基本相同,表明CB处理对有丝分裂的影响可能主要是推迟有丝分裂的启动。在G2晚期进行CB处理对有丝分裂启动的影响最为明显,说明在多头绒泡菌G2晚期可能存在着有丝分裂的检验点。Cytochalasin B, known as a functional inhibitor of actin, was microinjected into naturally synchronous plasmodia of Physarum polyecphalum, and the mitotic behaviours of both CB-treated specimens and the control were examined with light and electron microscopy. Mitosis in the CB-treated specimens began about 20 to 60 minutes later than that of the control. It was delayed 35 minutes in the specimens treated with CB in the S phase of the cell cycle, and the delayed bine was 20 minutes and 45 minutes, respectively. In the specimens treated with CB in early and middle G2 phase, the longest delay was 60 minutes found in the specimens treated in late G2 phase, indicating that mitosis was affected in Physarum polytcephalum when the function of actin was inhibited by CB treadment. The CB-treated specimens and the control showed similarities in the process of mitosis and dynamic changes of nuclear structures, suggesting that the main effect of CB treatment upon mitosis may be to delay the triggering of the mitosis.

关 键 词：肌动蛋白 细胞松弛素B 多头绒泡菌 有丝分裂 

分 类 号：Q343[生物学—遗传学]