马尾松针叶聚戊烯醇体外和体内抗氧化活性研究(英文)  

Antioxidant Activity of Polyprenols from Pine Needles of Pinus massoniana L. in vitro and in vivo

作　　者：郑光耀[1,2,3,4] 何玲[5] 高丽萍[1,2,3,4] 连燕霞[5] 谢衡 薄采颖[1,2,3,4] 李若达 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042 [5]中国药科大学药理学教研室,江苏南京210009 [6]中健行集团有限公司,广东乐昌512229

出　　处：《林产化学与工业》2013年第4期15-21,共7页Chemistry and Industry of Forest Products

基　　金：科技部农业科技成果转化资金项目(2008GB24320412);广东省重大科技专项(2012A080201005);'十二五'国家重大新药创制专项(2012ZX09504001-001)

摘　　要：研究马尾松针叶聚戊烯醇体外和体内的抗氧化活性,体外测定其对羟自由基(·OH)、超氧阴离子自由基(O2-·)和二苯基苦基苯肼自由基(DPPH·)的清除能力,以及对肝匀浆和肝线粒体自发性及Fe2+诱导性脂质过氧化的抑制作用,体内测定大鼠血清、肝匀浆和心匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量变化。结果表明:马尾松针叶聚戊烯醇能够有效抑制大鼠肝匀浆和肝线粒体脂质过氧化及肝线粒体肿胀程度,清除·OH、O-2.和DPPH·半数抑制浓度(IC50)分别为34.87、27.13、58.23 mg/L,增强大鼠血清、肝脏和心脏中SOD、GSH-Px活性和降低MDA含量(p<0.05)。马尾松针叶聚戊烯醇在体外和体内具有明显的抗氧化活性,为马尾松针叶聚戊烯醇在药物中的应用提供依据。The present study was undertaken to evaluate the antioxidant activity of polyprenols from pine needles of Pinus masso- niana L. in vitro and in vivo. In vitro antioxidant activities of polyprenols were evaluated by determining their ability to scavenge free radicals and inhibit lipid peroxidation and mitoehondrial swelling. In vivo antioxidant activities were determined by their effect on antioxidant enzymatic activities in serum, heart, and liver. The results showed that polyprenols exhibited concentration- dependent inhibition of lipid peroxidation and mitochondrial swelling at the 12.5 - 100 mg/L. It displayed high scavenging ca- pacity towards the hydroxyl, superoxide and DPPH radicals in a dose-dependent manner and the median inhibitory concentration （ICs0） were 34.87, 27.13 and 58.23 mg/L, respectively. Moreover, polyprenols could significantly increase the activities of SOD and GSH-Px while decrease the formation of MDA in serum, liver and heart （P〈0.05 ）. Polyprenols from P. massoniana L. have excellent antioxidant activities and might be a valuable antioxidant of natural source applicable in the medicine industry.

关 键 词：松针 聚戊烯醇 抗氧化 脂质过氧化 清除自由基 

分 类 号：TQ35[化学工程] R285.5[医药卫生—中药学]