Cd胁迫下马蔺根和叶中非蛋白巯基肽含量的变化  

The change of non-protein thiol content in roots and leaves of Iris lactea var. chinensis under Cd stress

作　　者：原海燕[1] 黄钢[1] 佟海英[1] 黄苏珍[1] 

机构地区：[1]江苏省中国科学院植物研究所南京中山植物园,江苏南京210014

出　　处：《生态环境学报》2013年第7期1214-1219,共6页Ecology and Environmental Sciences

基　　金：江苏省自然基金项目(BK2010477)

摘　　要：采用溶液培养,研究了Cd胁迫以及Cd胁迫下添加外源谷胱甘肽(GSH)和丁胱亚磺酰胺(BSO)对马蔺(Iris lacteavar.chinensis)根和叶干质量、Cd含量以及非蛋白巯基总肽(NPT)、谷胱甘肽(GSH)和其他非蛋白巯基化合物〔植物螯合肽(PC)、半胱氨酸(Cys)等〕含量的影响。结果表明:80 mg.L-1高质量浓度Cd胁迫下马蔺根系内Cd的大量积累显著抑制马蔺根系的生长,但相同Cd胁迫下添加100 mg.L-1GSH(PC合成底物)和BSO(PC合成抑制剂)后马蔺地上部Cd含量和根系干质量不同程度增加。根和叶中非蛋白巯基肽含量检测显示,与单独Cd胁迫相比,Cd胁迫下添加GSH和BSO对马蔺植物体内NPT总量基本无明显影响,而是对NPT、GSH和其他非蛋白巯基化合物在地上部和根系的分配产生一定影响,Cd胁迫下添加GSH后马蔺地上部NPT含量增加,而Cd胁迫下添加BSO后使得马蔺根系NPT含量增加。其中添加外源GSH促进了马蔺地上部GSH合成,使地上部GSH含量增加13.1%,其他非蛋白巯基化合物含量也增加;而添加BSO抑制马蔺地上部GSH合成,使地上部GSH含量降低7.1%,其他非蛋白巯基化合物含量也减少。但Cd胁迫下添加GSH和BSO后马蔺根系GSH含量均显著增加,且绝大部分GSH分布于马蔺的根系组织,尤其Cd溶液中添加BSO后马蔺根系GSH含量约是地上部GSH含量的3.4倍,而Cd胁迫下添加BSO使马蔺地上部和根系其它非蛋白巯基化合物含量均出现不同程度下降。综合分析Cd胁迫下添加外源GSH和BSO后马蔺干质量、Cd含量以及不同非蛋白巯基肽含量的变化及关系表明Cd胁迫下GSH较PC在马蔺Cd解毒和转运中具有更重要的作用。The effects of cadmium（Cd） stress and exogenous GSH（Glutathione） and BSO [L-buthione（S,R） sulfoximine] application on dry biomass,Cd content and the contents of NPT（total non-protein thiol）,GSH and other non-protein thiol [PC（phytochelatin）,Cys（cystic）,etc.] in roots and leaves of Iris lactea var.chinensis were studied by hydroponic culture method.The results showed that the massive accumulation of Cd significantly inhibited the growth of root in Iris lactea var.chinensis,but aboveground Cd content and dry biomass of root of Iris lactea var.chinensis increased in degrees when adding 100 mg.L-1 GSH（PC synthetic substrate） and BSO（PC synthesis inhibitor） to the same Cd solution.The detection results of non-protein thiol peptide content in roots and leaves showed that the GSH and BSO application had no significant impact on NPT content in Iris lactea var.chinensis under Cd stress,but a certain impact on the distribution of NPT,GSH and other non-protein thiol compounds in shoots and roots.The NPT content in the shoot of Iris lactea var.chinensis increased under adding GSH in Cd solution,while NPT content in the root increased when adding BSO to Cd solution.The exogenous GSH application promoted the synthesis of GSH and increased by 13.1% in shoot of Iris lactea var.chinensis,also increased the contents of other non-protein thiol compounds.However,exogenous BSO application inhibited the synthesis of GSH and decreased by 7.1%,also decreased the contents of other non-protein thiol compounds.The GSH contents in roots increased significantly and mainly located in root of Iris lactea var.chinensis when GSH and BSO application to the Cd solution.Furthermore,the root GSH contents were about 3.4 times higher than which in the shoot of Iris lactea var.chinensis when adding 100 mg.L-1 BSO in Cd solution,but the contents of other non-protein thiol compounds in shoots and roots decreased in the same time.Therefore,through a comprehensive analysis on the changes and relationship among dry biomass,Cd

关 键 词：CD胁迫 马蔺 非蛋白巯基 谷胱甘肽 植物螯合肽 

分 类 号：X171.5[环境科学与工程—环境科学]