长链非编码RNA SPRY4-IT1在食管鳞癌中的表达及对细胞生长的影响  

Long non-coding RNA SPRY4-IT1 expression in esophageal squamous cell carcinoma and its effects on cell growth

作　　者：谢海伟[1] 陈仿军[1] 朱斌[1] 曹刚[1] 金磊[1] 周国志[1] 吕进[1] 曹秀峰[1] 

机构地区：[1]南京医科大学附属南京医院肿瘤外科,南京市210006

出　　处：《中国肿瘤临床》2013年第17期1011-1015,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金青年项目(编号:81201881);南京市科委科技发展项目(编号:201108027)资助~~

摘　　要：目的:探讨长链非编码RNA SPRY4-IT1与食管鳞状细胞癌(ESCC)的临床病理及预后的相关性,以及对细胞生长的影响。方法:收集2008年1月至2009年12月南京医科大学附属南京医院肿瘤外科50例ESCC手术切除标本(包括癌组织和癌旁组织),荧光实时定量PCR(qRT-PCR)检测50例ESCC中SPRY4-IT1的表达情况,分析其与临床病理及预后的关系,用小干扰RNA(siRNA)干扰SPRY4-IT1后MTT法检测其对细胞生长的影响,流式细胞检测对细胞凋亡和周期的影响。结果:45例(90%)标本中SPRY4-IT1呈阳性表达,SPRY4-IT1在癌组织中的表达量显著高于癌旁组织(t=5.377,P<0.01)。SPRY4-IT1的相对表达水平与肿瘤大小、临床分期相关(P均<0.05)。SPRY4-IT1在ESCC细胞株中表达量高于正常食管上皮细胞。KYSE30细胞中干扰SPRY4-IT1的表达后可明显减慢细胞生长,阻滞细胞周期并促进细胞凋亡(P<0.01)。结论:SPRY4-IT1在ESCC组织中显著高表达,并且能促进细胞生长,可能成为诊断和判断预后的重要分子标记物。Objective：This study aimed to clarify the correlation of SPRY4-IT1 expression with the clinicopathological character-istics and prognosis of patients with esophageal squamous cell carcinoma （ESCC）, as well as the role of SPRY4-IT1 in promoting ES-CC cell growth. Methods：Quantitative real-time polymerase chain reaction for SPRY4-IT1 expression was performed on 50 paired can-cerous and adjacent non-cancerous esophageal specimens. Small interfering RNA was used to suppress SPRY4-IT1 expression to fur-ther explore its role in tumor progression. Cell viability was tested in vitro by MTT assay （OD=490 nm）, and cell apoptosis and cell cy-cle were investigated by flow cytometry. Results：We found markedly elevated SPRY4-IT1 expression in cancerous tissues compared with adjacent non-cancerous tissues （90%, P0.05）. Further experiments showed that SPRY4-IT1 expression levels were significantly higher in three ESCC cell lines than in the normal human esophageal epithelial cell line Het-1A. In vitro assays of the ESCC cell line KYSE30 demonstrated that knockdown of SPRY4-IT1 expression by small interfering RNA reduced cell growth, mediated cell cycle arrest at the G0-G1 phase, and promoted cell apoptosis （all P〈0.01）. Conclusion：SPRY4-IT1 was overexpressed in ESCC tissues and ESCC cell lines and promoted the growth of ESCC cells. The dysregulated expression of long non-coding RNA SPRY4-IT1 may play an important role in the process of ESCC development and may be developed as a useful biomarker for the diagnosis and prognosis of ESCC.

关 键 词：食管鳞癌 长链非编码RNA SPRY4-IT1 增殖 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]