人源电压门控质子通道对乳腺癌细胞迁移与侵袭的影响  

Effects of voltage-gated proton channel (Hv1) on the migration and invasion of breast cancer cells

作　　者：王雅蕾[1] 张尚荣[2] 王一凡[2] 孙保存[1] 李树杰[2] 

机构地区：[1]天津医科大学肿瘤医院病理科,国家肿瘤临床医学研究中心,乳腺癌防治教育部重点实验室,天津市300060 [2]南开大学物理科学学院

出　　处：《中国肿瘤临床》2013年第17期1025-1028,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目(编号:30970579;31271464)资助~~

摘　　要：目的:明确人源电压门控质子通道蛋白(human voltage-gated proton channel 1,Hv1)对乳腺癌细胞迁移及侵袭能力的影响。方法:检测Hv1在不同转移能力的人乳腺癌细胞系中的表达,利用小RNA干扰(siRNA)技术下调Hv1在乳腺癌MDA-MB-231细胞中的表达,通过细胞划痕和体外侵袭实验方法,观察Hv1对乳腺癌细胞迁移和侵袭的影响并初步探讨相关分子机制。结果:Hv1在高转移的乳腺癌细胞MDA-MB-231中表达较高,Hv1基因的siRNA干扰片段能够抑制Hv1基因及蛋白的表达;细胞划痕和体外侵袭实验表明Hv1降表达的MDA-MB-231细胞迁移和侵袭能力较弱;明胶酶谱和免疫印迹实验证明下调基因在细胞中的表达明显抑制了的活性。结论:能够促进乳腺癌细胞迁移及侵袭。Objective：To clarify the effect of voltage-gated proton channel 1 （Hv1） on the migration and invasion of breast cancer cells. Methods：The protein expression of Hv1 was detected in human breast cancer cell lines with different metastatic abilities. SiRNA technique was used to down-regulate the expression of Hvl in breast cancer MDA-MB-231 cells. Scratch and matrigel invasion methods were used to observe the effect of Hvl on the migration and invasion of breast cancer cells, and the relevant molecular mechanism was explored. Results：Hv1 was highly expressed in the highly metastatic breast cancer cell line MDA-MB-231. Hvl was more highly expressed in MDA-MB-231 cells with higher metastatic ability. The SiRNA sequence target at Hvl inhibited Hvl expression. Scratch and matrigel invasion experiments showed that the migration and invasion of MDA-MB-231 cells were significantly attenuated when Hv1 was knocked down by siRNA targeting Hv1. Zymography experiment on matrix metalloproteinase indicated that the enzyme activities of MMP-2 markedly decreased. Conclusion：Hv1 promoted the migration and invasion ability of breast cancer cells.

关 键 词：电压门控质子通道 乳腺癌 SIRNA 迁移 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]