CD133阳性和阴性胶质瘤U87细胞基因组学分析  

Genomics analysis of CD133+and CD133-cells derived from glioma U87cells

作　　者：王存祖 陆晓峰[2] 赵鹏[3] 尤永平[3] 傅震[3] 欧阳琦 向定朝[2] 徐敏[2] 

机构地区：[1]苏北人民医院神经外科,江苏省225001 [2]江苏大学临床医学院 [3]南京医科大学第一附属医院神经外科 [4]基础医学与医学技术学院

出　　处：《江苏医药》2013年第16期1864-1867,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2005156);江苏省卫生厅面上科研项目(H2006040);镇江市重点医学人才项目

摘　　要：目的分析胶质瘤U87中的CD133阳性(CD133+)和阴性(CD133-)细胞,建立差异性基因文库。方法通过磁珠选择分离U87细胞中的CD133+和CD133-细胞,利用AgilentHuman 1Aoligo芯片检测2种细胞总体基因,分析结果,建立差异性基因文库。结果基因组学研究中,CD133+和CD133-U87细胞比较,差异性基因数为840个;其中,上调基因759个,下调基因81个。在各基因功能分组中,与翻译调控功能相关的基因,差异性基因所占比率最大,为14.9%;各功能组之间差异性基因比率均有统计学意义(P<0.01)。结论通过基因组学研究,建立了CD133+和CD133-U87细胞差异性基因文库,为进一步研究脑肿瘤干细胞提供了线索。Objective To analyze the CD133＋ and CD133- cells in glioma U87 cells for establishing a differencial gene bank. Methods The CD133＋ and CD133- U87 cells were isolated by magnetic cell sorting. The total RNA of the cells was extracted from CD133＋ and CD133- U87 cells, respectively, which was analyzed with a DNA microarray（Agilent Human 1A oligo）. The differential genes were screened out by software analysis. Results A total of 840 differential expression genes was identified, whivh includeed 759 upregulated genes and 81 downregulated genes. Among those the ratio of differential expression genes in translation-regulator-activity group was 14. 9 %, the highest in all gene ontology groups（P〈0. 01）. Conclusion Differentially expressed genes have been obtained by genomics analysis,which are important targets for the study of brain tumor stem cells.

关 键 词：胶质瘤 CD133阳性细胞 基因组学 

分 类 号：R739[医药卫生—肿瘤]