颈髓后角环氧化酶2在偏头痛大鼠中枢敏化中的表达  

Expression of cyclooxygenase-2in posterior horn of cervical spinal cord in rat model with migraine pain hypersensitivity

作　　者：李军[1] 黄琳 郭永涛 武茜 史兆春 龚洁 沈飞飞 燕兰云 万琪 

机构地区：[1]南京医科大学第一附属医院神经内科,江苏省210029

出　　处：《江苏医药》2013年第16期1868-1871,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81070896)

摘　　要：目的探讨实验性偏头痛大鼠痛觉超敏行为及颈髓C1、C2后角环氧化酶2(COX-2)在偏头痛大鼠中枢敏化过程中的表达。方法健康成年SD大鼠30只,随机均分五组:空白对照(A)组、假手术(B)组、生理盐水(C)组、新型致炎剂(NIS)4d(D)组和NIS 7d(E)组。用Von-Frey纤维丝测定各组动物眶周痛觉阈值,免疫组化、Western blot检测大鼠颈髓C1、C2后角COX-2表达的变化。结果 C组大鼠的疼痛行为与A组相仿(P>0.05)。E组大鼠建模后第4天眶周痛觉阈值明显低于A组(P<0.05)。D组、E组COX-2阳性细胞计数均高于C组(P<0.01)。E组大鼠颈髓后角COX-2蛋白表达量明显高于A组(P<0.05)。结论 NIS硬脑膜给药能有效诱导偏头痛大鼠产生痛觉超敏,并与大鼠颈髓后角COX-2表达的增加密切相关。Objective To observe the effects of new inflammatory soup （NIS） on the hypersensitivity behaviors in experimental rats with migraine and the expression of cyclooxygenase-2 （COX-2） in the posterior horn of cervical spinal cord. Methods Thirty male SD rats were equally randomized into 5 groups of A（blank control）, B（sham surgery B）, C（saline-treated）, D（on the 4th d after NIS-infused） and E（on the 7th d after NIS-infused）. Von-Frey hair was used to monitor the pain threshold of allodynia around orbital skin. The expression of COX-2 in cervical spinal cord posterior horn was detected by immunohistochemistry and Western blot. Results There was no significant difference in the hypersensitivity behaviors between groups of A and C（P〉0. 05）. The pain threshold of allodynia on the 4th d after NIS-infused was lower in group E than that in group A（P〈O. 05）. The number of COX-2 positive cells was more in groups of D and E than that in group C（P〈0. 01）. The expression of COX-2 protein in the cervical spinal cord posterior horn was higher in group E than that in group A（P〈0. 05）. Conclusion Dural matter infusion of NIS can induce mechanical allodynia in rats, which is associated with an increased expression of COX-2 in the posterior horn of cervical spinal cord.

关 键 词：偏头痛 痛觉超敏 环氧化酶2 新型致炎剂 

分 类 号：R747[医药卫生—神经病学与精神病学]