miR-218在上皮性卵巢癌细胞株SKOV3中的生物学功能  

A study on biological function of miR-218in epithelial ovarian cancer cell line SKOV3

作　　者：马晶晶 吴姗 石莉 万一聪 程文俊 

机构地区：[1]南京医科大学第一附属医院妇科,江苏省210029

出　　处：《江苏医药》2013年第16期1881-1884,F0002,共5页Jiangsu Medical Journal

基　　金：基金项目:江苏省科技厅自然科学基金组织面上项目(BK2010576);江苏省六大人才高峰(303070774IB09)

摘　　要：目的探讨微小RNA-218(miR-218)在上皮性卵巢癌细胞株SKOV3中发挥的生物学功能。方法采用实时定量PCR方法检测上皮性卵巢癌细胞株中miR-218的表达。通过转染miR-218模拟体、miR-218抑制剂外源性地增高或者降低miR-218在SKOV3中的表达,利用MTT、流式细胞术、癌细胞侵袭迁移实验观察miR-218对SKOV3增殖、凋亡和侵袭转移的作用。结果 miR-218能增强SKOV3增殖能力(P<0.05),而与细胞凋亡、侵袭转移无相关性(P>0.05)。结论 miR-218在上皮性卵巢癌细胞株SKOV3中发挥调节细胞增殖的作用。Objective To investigate the biological function of microRNA-218 （miR-218） in epithelial ovarian cancer cell line SKOV3. Methods Real time quantitative PCR was used to detect the expression of miR-218 in epithelial ovarian cancer cell line SKOV3. The effects of miR-218 on the proliferation, apoptosis, invasion and metastasis of epithelial ovarian cancer cell lines were observed by MTT,annexin V-FITC/PI double staining and Transwell methods after exogenously increasing or decreasing the expression of miR-218 in SKOV3 by transfecting miR-218 mimics or miR-218 inhibitor. Results miR-218 enhanced cell proliferation of SKOV3（P〈0. 05）, but had no effect on cell apoptosis,invasion and metastasis （P〉0. 05）. Conclusion miR-218 may play a role in the cell proliferation in epithelial ovarian cancer cell line SKOV3.

关 键 词：上皮性卵巢癌细胞株 SKOV3 微小RNA-218 

分 类 号：R737[医药卫生—肿瘤]