男性不育160例Y染色体基因微缺失分析  

An analysis on AZF micro-deletion in 160 male with infertility

作　　者：王洪华[1] 蔡立义[1] 俞红英[1] 熊芳[1] 杨炜敏[1] 宋晓庆[1] 邹廉[1] 周萍[1] 胡玲卿[1] 周小金[1] 

机构地区：[1]南京医科大学附属无锡妇幼保健院,江苏省214002

出　　处：《江苏医药》2013年第16期1915-1917,共3页Jiangsu Medical Journal

基　　金：无锡市医院管理中心重大项目(YGZZ1107)

摘　　要：目的探索男性不育与Y染色体基因微缺失之间的关系。方法采用PCR技术,针对124例严重少精子症(A组)、36例无精子症(B组)与41例已正常生育的男性(C组)进行AZFa、AZFb、AZFe 3个区域共12个序列标签位点的微缺失分析。结果 A组中发现Y染色体微缺失24例,B组发现12例,而C组未发现Y染色体微缺失。此研究中发现微缺失形式有两种,分别是AZFa+AZFb+AZFc区的全缺失和AZFc区的单独缺失。结论 Y染色体微缺失与精子发生障碍导致的不育有一定的联系。Objective To explore the relationship of the Y chromosome microdeletion and male infertility. Methods With multiplex PCR technique, the microdeletion in three areas of AZFa, AZFb and AZFc of 12 sequence tag sites （STS） was analyzed in 124 severe idiopathic oligozoospermia （group A）, 36 idiopathic azoospermia（group B）, and 41 healthy normal fertile male（group C）. Results The Y chromosome microdeletion was observed in 24 cases of group A, which was seen in 9 cases of group B, but not seen in group C. The patterns of Y chromosome microdeletion identified were AZFa ＋AZFb＋AZFc and AZFc. Conclusion Y chromosome microdeletion might be closely related to male infertility resulting from spermatogenic failure.

关 键 词：Y染色体微缺失 男性不育 

分 类 号：R698[医药卫生—泌尿科学]