脂肪间质干细胞通过SDF-1促进胰腺癌细胞增殖、侵袭的体外研究  

Adipose-derived Mesenchymal Stem Cells Promoted the Proliferation and Invasion of Pancreatic Cancer Cells by SDF-1 in Vitro

作　　者：俞富祥[1] 宋才鑫[1] 吴志伟[1] 朱千东[1] 季世强[2] 张启瑜[1] 

机构地区：[1]浙江省温州医科大学附属第一医院肝胆胰外科,温州325000 [2]上海第二军医大学附属东方肝胆外科医院肝外一科,上海200438

出　　处：《中国细胞生物学学报》2013年第9期1294-1299,共6页Chinese Journal of Cell Biology

基　　金：浙江省重中之重外科学组项目(批准号:浙教高科2008-255)资助的课题~~

摘　　要：探讨脂肪间质干细胞(adipose tissue-derived mesenchymal stem cells,ADSCs)对胰腺癌(pancreatic cancer,PaCa)细胞增殖、侵袭的影响及其产生作用的原因。从腹腔脂肪分离纯化培养ADSCs,通过半透膜在6孔塑料培养板上建立PaCa细胞与ADSCs的双层培养体系,单独培养的PaCa细胞作为对照。通过CCK-8比色法检测ADSCs对PaCa细胞增殖的影响;ELISA法测定培养液中基质细胞源性因子-1(SDF-1)的浓度;RT-PCR法测定PaCa细胞及ADSCs中CXCR4的表达;评估测定SDF-1对PaCa细胞增殖的影响;AMD3100对ADSCs与PaCa细胞共培养的影响。结果显示,ADSCs可促进PaCa细胞的增殖与侵袭;SDF-1在ADSCs中高表达而在PaCa细胞几乎不表达;相反,CXCR4mRNA在PaCa细胞中高表达,而在ADSCs中低表达;SDF-1对PaCa的促增殖作用呈浓度依赖性;AMD3100能降低ADSCs对PaCa活性的影响。ADSCs具有促进PaCa细胞增殖及侵袭的潜能,这种作用可能与SDF-1/CXCR4轴有关。The aim was to explore the effects of adipose tissue-derived stem cells （ADSCs） on the prolifer- ation and invasion of pancreatic cancer （PaCa） cells in vitro and the the possible mechanism involved. ADSCs were isolated and co-cultured with PaCa cells. CCK-8 assay was used to detect the proliferation of PaCa cells. An ELISA was used to determine the concentration of stromal cell-derived factor-1 （SDF-1） in the supernatants. RT-PCR was performed to detect the expression of SDF-1 and CXCR4 in PaCa cells and ADSCs. The proliferation of PaCa cells by SDF-1 was measured. AMD3100 regulated the activity of PaCa cells after co-culture of ADSCs and PaCa. ADSCs could promote the proliferation and invasion of PaCa cells; The expression of SDF-1 was high in ADSCs, but not in PaCa cells. On the contrary, Higher CXCR4 mRNA levels were detected in the PaCa cells compared toADSCs. The promotion of SDF-1 on PaCa cells depended on the concentration of SDF-1; AMD3100 significantly downregulated these growth-promoting effects of ADSCs on PaCa cells. ADSCs could promote the proliferation and invasion of PaCa cells, which might involve the SDF-1/CXCR4 axis.

关 键 词：脂肪间质干细胞 胰腺癌 增殖 侵袭 SDF-1 

分 类 号：R735.9[医药卫生—肿瘤]