酵母RNA聚合酶Ⅲ负调控因子Maf1的研究进展  

Research Progress on the Function of Maf1 as A Negative Regulator of RNA Polymerase Ⅲ in Yeast

作　　者：闫洪波[1] 蒋伶活[1,2] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]江南大学生物工程学院,粮食发酵工艺与技术国家工程实验室,无锡214122

出　　处：《中国细胞生物学学报》2013年第9期1412-1417,共6页Chinese Journal of Cell Biology

基　　金：江南大学自主科研计划–重点项目(批准号:JUSRP51313B)资助的课题~~

摘　　要：在生长受限条件下,由RNA聚合酶Ⅲ控制的tRNA合成是受抑制的,这种抑制是通过RNA聚合酶Ⅲ转录活性的负调节因子Maf1介导的。Maf1最先是从酿酒酵母中通过遗传学方法发现的,在酵母到人体的真核细胞中保守存在。Maf1的磷酸化状态决定它的亚细胞定位以及与RNA聚合酶Ⅲ复合体的相互作用。目前,多个Maf1蛋白激酶也已被证实参与了RNA聚合酶Ⅲ的活性调控。此外,Maf1还可间接影响tRNA的成熟与降解。该文主要就酵母中Maf1对于RNA聚合酶Ⅲ的活性调控机理及其在tRNA生物合成中的作用进行了概述。同时,探讨了目前Maf1研究中存在的问题及其未来的研究方向。The tRNA biosynthesis is down-regulated by RNA polymerase III （Pol III） under growth-limit- ing conditions in budding yeast, Saccharomyces cerevisiae. This repression is mediated by Mall, a negative regula- tor of Pol III transcription. Conserved from yeast to man, Marl was originally discovered in S. cerevisiae through a genetic approach. The phosphorylation status of Mafl determines its subcellular localization and interaction with the Pol III complex. Several Marl kinases have been identified to be involved in Pol III control. Moreover, Mafl indirectly affects tRNA maturation and decay. Here we summarize the current understanding of the mechanisms by which Mafl mediates Pol III activity and tRNA biosynthesis in yeast.

关 键 词：酿酒酵母 RNA聚合酶III Maf1 转移核糖核酸 

分 类 号：R341[医药卫生—基础医学] Q78[生物学—分子生物学]