生物泥浆反应器中微生物数量变化与PAHs降解  

THE VARIATION OF AMOUNT OF MICROBES AND ITS RELATIONS WITH THE DEGRADATION OF PAHs IN THE BIOSLURRY REACTOR

作　　者：许华夏[1] 宋玉芳[1] 井欣[2] 任丽萍[1] 

机构地区：[1]中国科学院沈阳应用生态所陆地生态系统痕量物质生态过程开放实验室 [2]国家环境保护局环境监测总站

出　　处：《应用与环境生物学报》2000年第5期452-456,共5页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院重大项目!(KZ95 1 B1 2 0 7 0 1 0 2 );国家自然科学基金!(No :2 99770 2 1);沈阳生态站课题资助

摘　　要：分别以浓度、温度、接种量、通气量和表面活性剂为降解调控因子 ,采用平板记数法进行了生物泥浆反应器中微生物数量变化与PAHs降解的关系研究 .结果表明 :土壤中细菌数量与PHE、PY投加浓度呈显著正相关 .PHE、PY初始浓度越高 ,细菌数量越大 .此外 ,反应器的温度对微生物生长速度和数量有重要影响 .反应器温度为 30℃时对细菌迅速繁殖有利 .反应器温度为 2 0℃时对真菌生长繁殖有利 .接种量为 5 %即可明显提高PHE和PY的降解率 .研究还表明 ,通气量与细菌数量明显负相关 ,与真菌数量及PHE和PY的降解率呈明显正相关 ,说明有氧条件有利于真菌生长 .真菌的数量和通气量调控对PHE和PY快速降解起重要作用 .表面活性剂投加量w =5 0～ 2 0 0mgkg-1时 ,可促进PHE和PY的降解 ,尤其对PY的降解作用更为明显 .表 8参Taking concentration, temperature, inoculation amount,aeration and surfactant as regulation factors and by plate account (CFU) method to account the microbes, the study on the variation of microbes amount and its relations with the degradation of PAHs in the bio-slurry reactor is conducted. Results indicated that the bacteria amount in soil was positive related with the concentration of phenanthrene and pyrene spiked. The higher the initial content of phenanthrene and pyrene in soil, the higher the bacteria in amount. In addition, temperature in reactor played an important role on the rapid growing of microbes. Temperature in 30℃ benefited the fast growing of bacteria and in 20℃ benefited the rapid growth of fungi. Inoculation amount in 5% can increase the degradation rate of phenanthrene and pyrene. This study also showed that aeration amount was negative related with the bacteria amount and positive related with fungi's as well as the degradation rates of phenanthrene and pyrene, which illustrated that aerated condition benefited the growing of fungi. Fungi and aeration's control played an important role for the rapid degradation of phenanthrene and pyrene. Surfactant spiked in the concentration of w =50～200 mg kg -1 can promote both the degradation of phenanthrene and pyrene, especially for pyrene. Tab 8, Ref 7

关 键 词：生物泥浆反应器 微生物 生物降解 调控因子 

分 类 号：X172[环境科学与工程—环境科学] X52