白藜芦醇对胶质瘤U87细胞MMP-2表达和活性水平的影响  

Effects of resveratrol on activity and expression of MMP-2 in glioma U87 cells

作　　者：熊伟[1] 黄惠勇[1] 章翔[1] 韩宁[1] 李晓明[1] 殷安安[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2013年第4期293-296,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30870843;30672663)

摘　　要：目的探讨白藜芦醇对胶质瘤U87细胞基质金属蛋白酶(MMP)-2表达和活性水平的影响。方法采用明胶酶谱实验和免疫印迹实验分别检测白藜芦醇对胶质瘤U87细胞MMP-2活性水平和蛋白表达水平的影响;细胞免疫化学实验检测白藜芦醇对U87细胞核因子kappa B(NF-κB)转录活性的影响。结果明胶酶谱实验显示,40μM白藜芦醇处理U87细胞4 h、24 h和48 h后,MMP-2的活性水平明显降低(P<0.05);免疫印迹实验证实,40μM白藜芦醇能够明显降低U87细胞MMP-2的蛋白表达水平(P<0.05);细胞免疫化学结果表明,经40μM白藜芦醇处理后,p65从胞浆向胞核的转位明显减少(P<0.05)。另外,应用NF-κB抑制剂SN50处理细胞后,MMP-2的蛋白表达水平和活性水平均下降(P<0.05)。结论 40μM白藜芦醇能够明显抑制U87细胞MMP-2的蛋白表达水平和活性水平,其机制可能与白藜芦醇抑制了NF-κB的转录活性相关。Objective To evaluate the effects of resveratrol （Res） on expression and activity of matrix- metalloproteinase （MMP）-2 in glioma U87 cells. Methods Activity and expression of MMP-2 in U87 cells were detected by gelatin zymography assay and Western blotting, respectively; irranunocytochemistry was performed to determine the effect of Res on transcriptional activity of nuclear factor （NF-κB）. Rasults As gelatin zymography assay showed, the activity of MMP-2 was decreased significantly in U87 cells treated by 40 μM Res for 4 h, 24 h and 48 h （P 〈 0. 05）. Western blotting confirmed Res-indueed inhibition of MMP-2 protein expression （ P 〈 0. 05 ）. Results from immunocytochemistry showed the translocation of p65 from cytoplasm to nucleus was blocked by 40 κM Res treatment （P〈0.05）. In addition, SN50, a NF-κB inhibitor, reduced both the activity and protein expression of MMP-2 in U87 cells （P 〈0.05）. Conclusion Activity and expression of MMP-2 can be inhibited by 40μM Res in U87 cells, which may be attributed to Res-induced suppression of NF-κB transcriptional activity.

关 键 词：白藜芦醇 胶质瘤 基质金属蛋白酶 NF-κB 

分 类 号：R730[医药卫生—肿瘤]