乳杆菌微胶囊化培养的研究  

CULTIVATION OF LACTOBACILLUS IN MICROCAPSULE

作　　者：叶子坚[1] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程系,杭州310027

出　　处：《微生物学报》2000年第5期507-512,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金!( 2 96760 3 9) ;国家教育部回国人员基金资助项目!( 1 997)&&

摘　　要：采用MaCS PDMDAAC微胶囊对两种乳杆菌进行了固定化发酵研究。结果表明 ,两种乳杆菌能够在微胶囊内很好地生长和繁殖 ,菌浓度可分别达到 1 8× 1 0 1 1 /mL胶囊和 2 69× 1 0 1 1 /mL胶囊 ,比游离培养高出十倍以上 ,并且能与游离发酵一样产生乳酸 ,糖耗时间可缩短 1 /3～ 2 /3。进行的多批次发酵显示了巨大的产酸能力。The NaCS PDMDAAC capsules were used to immobilize the two kinds of lactobacillus. The experiment results showed that two kinds of lactobacillus could grow well, and the densities of cells were 1 8×10 11 /mL eapsule and 2 79×10 11 /mL capsule, respectively which was much higher than that in free cell culture. The conversion rate of lactic acid could be 2～5 folds higher than that in free cell culture.

关 键 词：固定化细胞培养 乳酸发酵 乳杆菌 

分 类 号：Q814[生物学—生物工程]