中国春Ph1基因的分子标记及冬小麦ph1b代换系的获得  

Molecular Markers of Ph1 Gene in Chinese Spring and Development of Winter Wheat New Line Harboring ph1b Gene

作　　者：王新望[1] 赖菁茹[2] 陈梁鸿[3] 刘广田[3] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]河南省农业科学院小麦研究所,郑州450002 [3]中国农业大学植物遗传育种系,北京100094

出　　处：《Acta Botanica Sinica》2000年第3期274-278,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：利用大麦 (HordeumvulgareL .)第 5染色体上RFLP探针衍生的 19个序列标志位点PCR(STS_PCR)引物对“中国春”小麦 (TriticumaestivumL .) (CS)及其ph1b突变体基因组总DNA进行PCR扩增 ,筛选出Ph1基因的一个连锁标记 ,再用“中国春”第 5部分同源群缺体_四体系和CS×ph1b突变体F2 群体证明并定位于离Ph1基因近着丝点端 5 .7cM (centiMorgan)处。然后将该标记转换成特异的序列特征扩增区 (SCAR)标记。以“阿勃”5B缺体为桥梁亲本 ,冬小麦“京 411”为受体亲本 ,“中国春”ph1b突变体为供体亲本 ,进行三轮杂交和一轮自交 ,每一轮经减数分裂分析和SCAR标记的辅助选择 ,快速地筛选出了ph1b基因型 ,并选得一个冬小麦“京 411”的ph1b中间代换系。The genomic DNA of common wheat ( Triticum aestivum L.) “Chinese Spring” (CS) and its ph1b mutant were analyzed by using 19 sequence tagged site PCR (STS_PCR) primers, which derived from RFLP probes from barley ( Hordeum vulgare L.) chromosome 5H. One marker was identified on wheat chromosome 5BL, which is 5.7 cM (centiMorgan) proximal to Ph1 gene, using the CS homoeologous group 5 nullisomic_tetrasomic, ditelosomic 5BL line and an F 2 population from CS×ph1b mutant. This linked PCR marker was converted into a more specific sequence characterized amplified region (SCAR) marker. To obtain a new winter wheat line containing ph1b gene, the authors used a nullisomic 5B line of “Abbodanza” as a bridge parent and crossed respectively with the CS ph1b mutant (donor) and a winter wheat variety, “Jing 411” (recipient). The meiotic chromosome pairing was checked in the progeny of each cross, as well as using the marker_assistant selection of the SCAR marker identified for ph1b gene. After three inter_crossing and one selfing, a relatively stable ph1b substitution line of winter wheat with “Jing 411” background was obtained.

关 键 词：小麦 ph1b突变体 分子标记 ph1基因 

分 类 号：Q943[生物学—植物学]