EC9706细胞和人食管鳞状细胞癌组织中MAL基因启动子区的甲基化状态  

Methylation analysis of MAL gene promoter region in EC9706 cell and human esophageal squamous cell carcinoma tissue

作　　者：刘伯新[1] 郭长青[1] 王曼华[1] 王明荣[2] 

机构地区：[1]郑州大学第一附属医院消化内科,郑州450052 [2]中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《郑州大学学报（医学版）》2013年第4期440-443,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨人食管鳞状细胞癌(食管鳞癌)MAL基因启动子区的甲基化状态。方法:利用亚硫酸氢盐测序法检测人食管鳞癌细胞系EC9706中MAL基因启动子区CpG位点甲基化的发生情况。根据测序结果选用甲基化敏感性限制性内切酶酶切结合PCR技术进一步检测26例食管鳞癌组织及配对癌旁正常组织MAL基因启动子区的甲基化情况。结果:EC9706细胞中MAL基因启动子区检测出42个胞嘧啶位点的甲基化,占所有CpG位点的73.7%(42/57)。食管鳞癌组织中MAL基因的甲基化率为53.8%(14/26),远远高于配对癌旁正常组织的7.7%(2/26)(χ2=10.924,P<0.001)。不同临床病理特征食管鳞癌组织中MAL基因启动子区甲基化率差异均无统计学意义(P>0.05)。结论:MAL基因启动子区的甲基化可能是食管鳞癌发生的机制之一。Aim： To explore methylation status of MAL gene promoter region in human esophageal cancer. Methods： Bisulfite-sequencing was used to detect the methylation pattern of MAL gene promoter region in esophageal cancer cell line EC9706. Then the methylation pattern of MAL gene promoter region in esophageal cancer and matched para-cancerous tissue was further detected by methylation sensitive restriction endonuclease which was selected according to the sequencing results and PCR. Results： A total of 42 cytosine locuses were detected methylated in MAL gene promoter region in EC9706,accounting for 73.7% （ 42/57） of all CpG locuses. Methylation incidence of MAL gene in esophageal cancer was 53. 8% （14/26） ,much higher than 7.7% （2/26） in matched para-cancerous tissue（χ2=10. 924,P0.001） . Methylation of MAL gene promoter region in esophageal cancer with different clinicopathological features had no obvious significance（P0.05） . Conclusion： The methylation of MAL gene promoter region may be one of the possible mechanisms of the occurrence of esophageal cancer.

关 键 词：食管鳞状细胞癌 EC9706细胞 MAL基因 启动子区甲基化 

分 类 号：R735.1[医药卫生—肿瘤]