阿尔茨海默病患者血液标本外吐小体中miRNA-135a的检测  

Detection of miRNA-135a in Exosome of Blood Samples from Patients with Alzheimer's Disease

作　　者：刘辰庚[1] 王金玲[1] 李蕾[1] 张雁冰[1] 宋静[1] 洪萍[1] 王培昌[1] 

机构地区：[1]首都医科大学宣武医院检验科,北京100053

出　　处：《现代生物医学进展》2013年第22期4361-4363,4287,共4页Progress in Modern Biomedicine

基　　金：教育部博士点基金项目(20121107110001)

摘　　要：目的:提取轻度认知障碍(MCI)、阿尔茨海默型痴呆(DAT)患者血液标本以及PC-12细胞培养基标本中的外吐小体并检测其中miRNA-135a含量。方法:使用超速离心法分离7例MCI、18例DAT患者、健康对照组的血清和血浆及PC-12细胞培养基中的外吐小体,使用实时定量PCR检测外吐小体中miRNA-135a的含量。结果:在PC-12细胞培养基、血清和血浆标本外吐小体中检出了miRNA-135a。MCI和DAT患者血清、血浆外吐小体miRNA-135a水平显著低于健康对照组(P<0.05),但MCI患者血清、血浆外吐小体miRNA-135a水平与DAT患者相比无显著性差异(P>0.05)。MCI、DAT和健康对照三者的血清和血浆标本外吐小体miRNA-135a水平均无相关性(P>0.05)。结论:本研究证实了血液和脑脊液标本外吐小体中存在miRNA-135a,对将外吐小体miRNA作为AD诊疗潜在标志物提供了证据。Objective： The exosomes of PC-12 cell mediums and blood samples from patients with mild cognitive impairment（MCI） and dementia of Alzheimer type（DAT） were extracted,and level of microRNA-135a（miRNA-135a） in the exosomes were determined.Methods： Extract the exosome of PC-12 cell mediums and blood samples from 7 MCI patients and 18 DAT patients and health control using ultracentrifugation method and then determine the level of miRNA-135a using real-time PCR.Results： MiRNA-135a were detected in PC-12 medium,serum and plasma.The levels of miRNA-135a in exosome from the serum and plasma of MCI and DAT patients were significantly lower than that of control group（P0.05）,while the level of miRNA-135a in exosome from the serum and plasma of MCI patients were similar with the DAT group（P0.05）.No correlation was observed between the levels of miRNA-135a in exosome of the serum and plasma from MCI and DAT patients（P0.05）.Conclusion： The miRNA-135a exists in the exosome from blood and CSF.It is suggested that the miRNA detection of exosome may have potential value in the diagnosis of Alzheimer＇s disease.

关 键 词：外吐小体 阿尔茨海默病 超速离心 microRNA-135a 

分 类 号：R74[医药卫生—神经病学与精神病学]