C57/BL6小鼠急性心肌梗死模型制备方法的总结与改良  

Preparation of C57/BL6 mice myocardial infarction model and method of improving survival rate

作　　者：盛瑾[1] 夏宇[2] 朱铠[3] 过常发[3] 王春生[3] 

机构地区：[1]遵义医学院附属医院心血管内科,贵州遵义563003 [2]遵义医学院附属医院胸心血管外科,贵州遵义563003 [3]上海复旦大学中山医院心血管外科,上海200032

出　　处：《贵州医药》2013年第3期195-197,共3页Guizhou Medical Journal

摘　　要：目的对C57/BL6小鼠急性心肌梗死模型建立方法进行总结和改良,以提高建模可靠性、稳定性及可重复性,减少死亡率。方法40只C57/BL6小鼠,腹腔内注射苯巴比妥钠全身麻醉后气管切开并插管,小动物呼吸机辅助呼吸。常规消毒铺巾后,切开左胸前区皮肤,经左侧第3、4肋间进入胸腔暴露心脏,左前降支冠状动脉结扎造成急性心肌梗死。对围手术期可导致失败和死亡的原因如麻醉、人工辅助呼吸、开胸过程及结扎冠状动脉位置等进行分析和改进。结果成功建立了C57/BL6小鼠急性心肌梗死模型。术后24hC57/BL6小鼠存活率为95%,4周时存活率为92.5%。结论通过方法改进,能快速有效地建立C57/BL6小鼠急性心肌梗死模型,并降低建模过程中C57/BL6小鼠的死亡率。Objective To investigate how to develop a credible, steady and repeatable rat model of acute myocardial infarction with a low mortality, improve the method and decreased those factors that influence death rate of creating the model. Methods 40 C57/BL6 mice were anesthetized by intraperitoheal injection of pentobarbital sodium. After tracheotomy intubations, respiration machine was linked. Left anterior thoracotomy through 3, 4 intercostal region was performed, and then the left anterior descending coronary arteries were ligated to produce acute myocardial infarction. During the operation, some details to affect rat mortality were analyzed and improved, which involving anesthesia, artificial respiration, thoracotomy and area of ligating the left anterior descending coronary artery, etc. Results The model of acute myocardial infarction was established successfully. Following the details were highlighted, survival rate was 95% after operation and 92.5% beyond three weeks. Conclusion C57/ BL6 mice acute myocardial infarction model can be made quickly by this way and the model＇s survival rate was improved effectively.

关 键 词：急性心肌梗塞 动物模型 C57 BL6小鼠 存活率 

分 类 号：R-322[医药卫生] R542.22