钙调神经磷酸酶在人外周血内皮祖细胞增殖调节中的作用  

Effect of calcineurin on regulating the proliferation of endothelial progenitor cells

作　　者：李隽[1] 何炯红[1] 杨龙[1] 覃智芳[1] 

机构地区：[1]贵州省人民医院心内科,贵州贵阳550002

出　　处：《贵州医药》2013年第4期296-299,共4页Guizhou Medical Journal

基　　金：贵州省科学技术基金资助项目[黔科合J字(2008)2309号]

摘　　要：目的观察钙调神经磷酸酶(CaN)活性改变对培养的人外周血内皮祖细胞(EPCs)增殖和凋亡的影响。方法密度梯度离心法分离外周血单个核细胞,贴壁培养获得EPCs。依据干预方式分四组:对照组;苯肾上腺素(PE,10μmol/L)组;环孢素A(CsA,1μg/mL)组;CsA+PE组。比色法测定CaN活性和细胞增殖能力;TUNEL染色检测细胞凋亡。结果 PE干预能明显增加细胞CaN活性,促进细胞增殖能力;CsA作用可明显抑制PE促进的CaN活性和细胞增殖,增加EPCs凋亡率。结论在培养的人外周血来源EPCs,CaN活性的维持具有促进细胞增殖和防止细胞凋亡的作用。Objective To investigate the role of calcineurin on regulating the proliferation and ap- optosis in cultured human endothelial progenitor cells （EPCs）. Methods EPCs were obtained from cultured mononuclear cells isolated from peripheral blood of healthy adults, and were divided into the following four groups depending on the different treatments administered： control （without agent）, phenylephrine （PE, 10μmol/L）, cyclosporine （CsA, 1 μg/mL）, and CsA＋PE （incubation with CsA for 1 h before PE was enrolled in） groups. CaN enzymatic activity and cell proliferation were determined using the colorimetrie method. Cell apoptosis was determined using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） staining. Results Stimulation with PE （10 μmol/L） potently promoted the activity of CaN enzyme and cell proliferation in EPCs that were attenuated by pretreatment with CsA （1 μg/ml）. Treatment with CsA potently suppressed the proliferation and increased the apoptotic rate of EPCs in the presence and absence of PE. Conclusion CaN play an im- portant role in promoting proliferation and inhibiting apoptosis in cultured human EPCs from peripheral blood.

关 键 词：钙调神经磷酸酶 内皮祖细胞 细胞增殖 细胞凋亡 

分 类 号：R-33[医药卫生] R329.25