1株颉颃放线菌的分离及抑菌活性的初步研究  

Study on the Isolation of a Strain of Antagonistic actinomycetes and Its Antibacterial Activity

作　　者：丁朋晓[1] 张连英[1] 曾庆华[1] 

机构地区：[1]遵义医药高等专科学校,贵州遵义563002

出　　处：《畜牧与饲料科学》2013年第7期20-22,共3页Animal Husbandry and Feed Science

摘　　要：从土壤中分离筛选到1株对多种病原性细菌具有抑菌作用的颉颃放线菌T12,并综合形态学特征、生理生化特征对其进行初步鉴定,测定其发酵液的稳定性。结果表明,T12对革兰氏阳性球菌的抑菌效果较好;在70℃以上,随着温度升高,发酵液抑菌活性略微减弱,但在100℃时仍表现出一定的抑菌活性,发酵液对温度的稳定性较强;在pH值小于6的条件下,随着pH值的降低发酵液抑菌活性减弱,但其对碱的稳定性较强。A strain of Antagonistic actinmycete T12 with bacteriostatic effects on several kinds of pathogenic bacteria was isolated from soil sample. And it was preliminarily identified by morphological characteristics, biological and physical characteristics. The stability of its fermentation liquid was determined. The results showed that T12 had better antibacterial effects on Gram-positive bacteria. At above 70 ℃, the antibacterial activity of the fermentation liquid was slightly weakened with the rise of temperature. And it still showed some antibacterial activity at 100 ℃. The fermentation liquid had stronger stability to temperature. When pH was lower than 6, the antibacterial activity of the fermentation liquid decreased with the reduce of pH, but it showed stronger stability to alkali.

关 键 词：颉颃放线菌 发酵液 稳定性 

分 类 号：S482.292[农业科学—农药学]