响应面法优化超声波辅助提取狭叶荨麻黄酮和木脂素工艺  

OPTIMIZATION OF ULTRASONIC-ASSISTED EXTRACTION OF FLAVONOIDS AND LIGNANS FROM URTICA ANGUSTIFOLIA BY RESPONSE SURFACE METHOD

作　　者：杜宏涛[1] 廖庆华[2] 曹勇[2] 徐军[2] 徐传柄[2] 王俊儒[1] 

机构地区：[1]西北农林科技大学理学院,陕西杨凌712100 [2]西北农林科技大学创新学院,陕西杨凌712100

出　　处：《北京师范大学学报（自然科学版）》2013年第4期360-364,共5页Journal of Beijing Normal University(Natural Science)

基　　金：教育部新世纪优秀人才支持计划资助项目(NCET-05-0855);国家级大学生创新性试验计划项目(2010GZ-4)

摘　　要：通过响应面法优化超声波提取狭叶荨麻中黄酮和木脂素的工艺,以黄酮和木脂素得率为指标,在考察乙醇浓度、提取时间、提取温度、提取次数和料液比的基础上,利用响应面法进行3因素3水平的试验.结果表明:最优条件是乙醇质量分数为87.62%、提取温度为54.15℃、料液质量体积比为1∶45.64(mg∶mL)、提取时间50min、提取3次;在该条件下,黄酮提取率10.875mg.g-1,木脂素提取率1.100mg.g-1;证实响应面法在同时提取2种以上中药有效成分时可以得到较为理想的结果.To optimize ultrasonic extraction conditions of flavonoids and lignans from Urtica angustifolia by response surface method.Ethanol concentration,ultrasonic time,ultrasonic temperature,ultrasonic time and liquid-solid ratio of single factor experiment was altered for 3 factors 3 levels response surface methodology.Optimized extraction conditions were determined as follows：ethanol concentration 87.62%,ultrasonic temperature 54.15℃,ratio of solvent volume 45.64∶1（mL∶mg）,ultrasonic time 50 min,extract runs 3.Under such conditions,extraction yield of flavonoids and lignans were 10.875 mg·g-1 and 1.100 mg·g-1,respectively.It is concluded that when two components extracted simultaneously from herb,central composite design-response surface optimization can get better results.

关 键 词：狭叶荨麻 黄酮 木脂素 响应面法 超声提取 

分 类 号：TS262.2[轻工技术与工程—发酵工程]