低氘水对肝癌HepG2细胞增殖和侵袭能力的抑制作用  

Deuterium-depleted water inhibits proliferation and invasion of HepG2 cells

作　　者：王坤[1] 祝葆华[1] 王宏强[2] 张力[1] 李婕[1] 陈淼[1] 张瀚彬[1] 邬永富[1] 蔡晶[3] 杨慧龄[1] 

机构地区：[1]广东医学院,广东东莞523808 [2]南华大学药物药理研究所,湖南衡阳421001 [3]武汉大学人民医院,湖北武汉430060

出　　处：《广东医学院学报》2013年第3期241-244,共4页Journal of Guangdong Medical College

基　　金：国家自然科学基金(No.30971438;81272334);广东省高等学校引进人才项目(No.XG1007);广东医学院博士基金(No.GK1005);东莞市高等院校科研机构科技计划重点项目[No.东科(2012)123号]

摘　　要：目的探讨低氘水(deuterium-depleted water,DDW)对肝癌HepG2细胞增殖、侵袭能力的抑制作用。方法 HepG2细胞及正常肝细胞LO2用50、75、100、150ppm DDW(纯净水对照组)处理,用MTT法、平皿克隆实验检测细胞增殖、侵袭转移能力,Western blot检测增殖细胞核抗原(PCNA)表达。结果 100ppm DDW处理24h对LO2细胞、72h对HepG2增殖抑制无影响(P>0.05)。HepG2在50、75ppm DDW中细胞克隆数均低于对照组,而LO2细胞克隆数高于对照组(P<0.01)。50、75、100ppm DDW处理后HepG2细胞PCNA表达明显低于对照组(P<0.01)。结论 DDW可抑制HepG2细胞增殖和侵袭能力,但促进正常细胞LO2生长,这可能与PCNA表达下调有关。Objective To investigate the inhibition of deuterium-depleted water （DDW） in the proliferation and invasion of HepG2 cells. Methods HepG2 ceils and normal heptocytes LO2 were treated with 50, 75, 100, 150 ppm DDW （pure water, control group）. The proliferation, invasiveness and PCNA expression of HepG2 and LO2 cells were determined using MTT, plate cloning assay and Western blot, respectively. Results Proliferation of LO2 cells and HepG2 ceils showed no change after 24 h and 72 h of 100 ppm DDW treatment （P〉0.05）, respectively. Compared with control group, colony formation decreased in HepG2 cells but increased in LO2 ceils after incubation with 50, 75 ppm DDW （P〈0.01）, and PCNA expression of HepG2 ceils reduced significantly after treatment with 50,75,100 ppm DDW （P〈0.01）. Conclusion DDW can inhibit the proliferation and invasion of HepG2 cells but reinforce the growth of LO2 cells, which may be related to the PCNA downexpression.

关 键 词：低氘水 HEPG2细胞 LO2细胞 增殖 PCNA 

分 类 号：R735.7[医药卫生—肿瘤]