腺病毒介导的SD-HA融合蛋白表达对慢性粒细胞白血病细胞增殖和凋亡的影响  

The impact of fusion protein SD-HA expression on cell proliferation and apoptosis in chronic myelocytic leukemia cell mediated by adenovirus

作　　者：彭智[1,2] 桂敏[1,2] 冯文莉[3] 杜莉[1,2] 

机构地区：[1]重庆医科大学附属第二医院 [2]<中华肝脏病杂志>编辑部,重庆400010 [3]重庆医科大学检验医学院,重庆400016

出　　处：《国际检验医学杂志》2013年第15期1922-1924,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨腺病毒介导的SD-HA融合蛋白表达对慢性粒细胞白血病(CML)细胞增殖和凋亡的影响。方法外源性融合蛋白表达的检测采用Westernblot;细胞经免疫荧光染色后用激光共聚焦显微镜观察SD-HA融合蛋白在细胞中的定位;采用MTT法检测细胞增殖的情况;采用流式细胞仪检测细胞周期和细胞凋亡。结果 SD-HA、SmD-HA融合蛋白能在CML细胞株中有效表达,且定位于胞浆。重组腺病毒Ad5F35-SD-HA感染CML细胞后能特异性阻滞CML细胞周期由G0/G1期向S期推进,具有抑制细胞的增殖效应,且对照组比较早期凋亡细胞明显增多(P<0.05)。结论腺病毒介导的SD-HA基因转染对CML细胞有抑制增殖和诱导凋亡的双重效应。Objeetive To explore the impact of SD-HA fusion peptide expression on ceils proliferation and apoptosis in chronic myeloid leukemia（CML） cells mediated by adenovirus. Methods Exogenous fusion protein expression was detected by Western blot. The localization of fusion protein SD-HA was observed through laser scanning eonfocal microscope after cell immunofluores- cence staining. Cell proliferation was determined by MTT test. Flow cytometry was employed in cell cycle and apoptosis detection. Results Adenoviral vector encoding SD-HA and StuD-HA were successfully constructed and validated by immunocytochemical staining and confocal microscopy. It was verified that recombinant adenovirus AdSF35-SD-HA have some inhibiting effects on cell proliferation after infection,such as blocking cell cycle from G0/G1 phase to S period. Flow cytometry tests showed that compared with control groups its early apoptotic cells were significantly increased（P〈0.05）. Conclusion Fusion protein SD-HA expressed in chronic myeloid leukemia cells mediated by adenovirus exhibits anti-proliferative and pro-apoptotic effects.

关 键 词：白血病 髓系 慢性 BCR-ABL阳性 含SH2域蛋白质酪氨酸磷酸酶类 细胞增殖 细胞凋亡 

分 类 号：R733.71[医药卫生—肿瘤]