溃疡性结肠炎患者血清自身抗体检测的临床价值  

Clinical significance of serum autoantibodies detection for patients with ulcer colitis

作　　者：谢文[1] 汪付兵[1] 余晓萍[1] 龙行华[1] 杨桂[1] 李瑞炎[1] 

机构地区：[1]武汉大学中南医院检验科,湖北武汉430071

出　　处：《国际检验医学杂志》2013年第15期1959-1960,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨血清核周型抗中性粒细胞胞浆抗体(P-ANCA)、抗小肠杯状细胞抗体(GAB)单独及两者联合检测对溃疡性结肠炎患者的临床价值。方法用间接免疫荧光法检测溃疡性结肠炎(UC)组(54例)和肠道疾病对照组(24例)及健康对照组(21例)人群血清P-ANCA和GAB,并将UC组患者按结肠镜下病变部位分为远端受累组(33例)和广泛结肠受累组(21例),分析各组患者血清P-ANCA和GAB检测阳性率的差异。结果 UC组、肠道疾病对照组和健康对照组的P-ANCA的阳性率分别为59.3%(32/54),8.3%(2/24)和0(0/21),各组之间差异有统计学意义(P<0.01);UC组患者的GAB阳性率为22.3%(12/54)高于肠道疾病对照组和健康对照组(P<0.05)。两者联合检测,UC组的阳性率为64.8%。结论 P-ANCA和GAB可作为UC的血清标志物,在UC的实验室诊断中具有重要的意义,联合检测P-ANCA和GAB可以提高诊断的阳性率。Objective To investigate the clinical significance of serum perinuclear anti-neutrophil cytoplasmic antibodies （P-AN- CA） ,anti-intestinal goblet cell antibodies （GAB） and their single or combined detection for patients with ulcerative colitis. Methods 54 patients with ulcerative colitis （UC）, 24 patients with bowel disease,and 21 healthy individuals were recruited in the study. P- ANCA and GAB in serums were detected by indirect immunofluorescence assay, and the UC group was divided into two groups, remote colon involvement group and extensive colon involvement group,according to colon＇s pathological changes with colonoscopy. The positive rates of serum P-ANCA and GAB were analysed. Results In UC group,bowel disease group and healthy group,the positive expression of P-ANCA were 59.3 % （32/54）, 8.3 % （2/24） and 0 （0/21）, respectively. Compared among those groups, there were significant difference（P〈0.01）. Similarly, positive expression of GAB in UC group, which was 22.3 % （12/54）, was higher than that in bowel disease group（0/24） and health group（0/21）（P〈0.05）. In the combined detection of P-ANCA and GAB, the positivity expression rate of UC group was 64. 8%. Conclusion The P-ANCA and GAB could be used as serum markers in the diagnosis of UC. Combined detection of P-ANCA and GAB can improve the positive rate of UC diagnosis.

关 键 词：结肠炎 溃疡性 抗体 抗中性白细胞胞质 抗体 抗小肠杯状细胞 

分 类 号：R574.62[医药卫生—消化系统] R446.6[医药卫生—内科学]