1种简单的白灵菇单孢分离新方法  

A New Method of Single Spore Isolation of Pleurotus eryngii var. tuoliensis

作　　者：郑素月[1] 卢月霞[1] 张庆桥[1] 

机构地区：[1]河北工程大学农学院,河北邯郸056038

出　　处：《中国食用菌》2013年第5期14-15,共2页Edible Fungi of China

基　　金：河北省科技支撑计划项目"白灵菇优质品种的杂交选育及评价";项目编号:11230908D-1-1

摘　　要：单孢分离是研究食用菌遗传育种的重要技术之一。介绍了1种简单、快速的利用琼脂块分离白灵菇单孢菌株的新方法。该方法不需特殊设备,操作简单、快速、准确,挑取的单核菌丝成功率达100%,值得推广到其它种类食用菌的单孢分离工作中。Single spore isolation was one of the important technology of edible fungus breeding. In this paper, a new simple, quick method was adopted to isolate the single spore of Pleurotus eryngii var. tuoliensis, which didn＇ t need special equipment and was simple, fast and accurate in operation. The success rate of mononuclear mycelia was 100%. This method was worth exten- ding to other edible fungi.

关 键 词：白灵菇 多孢分离 单孢分离 遗传育种 

分 类 号：S646.1[农业科学—蔬菜学]