桂皮醛诱导人胃癌BGC-823细胞凋亡及相关分子机制的探讨  

Discussion on Cell Apoptosis and Its Related Molecular Mechanisms of Human Gastric Carcinoma Cell Line BGC-823 Induced by Cinnamaldehyde

作　　者：冯程程[1] 邹玺[2] 吴坚[2] 周锦勇[2] 王瑞平[2] 

机构地区：[1]南京中医药大学第一临床医学院,南京210046 [2]江苏省中医院,南京210029

出　　处：《世界科学技术-中医药现代化》2013年第5期920-925,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江苏省人民政府办公厅江苏省高校优势学科建设工程资助项目(1006):中西医结合;负责人:刘沈林;江苏省卫生厅江苏省六大高峰人才项目(2012-WS-037):健脾扶正消癥方对晚期胃癌生长转移及血清miR-21表达的影响;负责人:邹玺;江苏省中医药局领军人才项目(LJ200908):健脾化瘀方干预大肠癌相关miRNAs表达的研究;负责人:王瑞平

摘　　要：目的:探讨桂皮醛对人胃癌BGC-823细胞增殖、凋亡及其相关分子机制。方法:采用MTT法检测桂皮醛对人胃癌BGC-823细胞增殖的影响;采用流式细胞仪检测桂皮醛诱导的凋亡,Hoechst 33342染色法观察凋亡形态的变化;RT-PCR法检测桂皮醛对人胃癌BGC-823细胞相关凋亡基因的影响;Western Bolt方法检测桂皮醛对人胃癌BGC-823细胞相关凋亡蛋白的影响。结果:与对照组相比,桂皮醛有抑制人胃癌BGC-823细胞增殖的作用(P<0.01);桂皮醛能诱导凋亡的产生;桂皮醛能下调凋亡相关基因Bcl-2、Bcl-xL、Survivin,上调凋亡相关基因Bax、Bak;桂皮醛能下调凋亡相关蛋白Bcl-2、Procaspase-3,上调凋亡相关蛋白Bax。结论:桂皮醛对人胃癌细胞BGC-823的增殖具有抑制作用并能诱导凋亡,可能与内源性凋亡途径的激活有关。This article was aimed to investigate the cell proliferation, cell apoptosis and its related molecular mechanisms of the human gastric carcinoma cell line BGC-823 in vitro after treatment with cinnamaldehyde. The MTT Assay demonstrated the inhibitory effect of cinnamaldehyde. And the Flow Cytometry was used to determine its induction of cell apoptosis. The Hoeehst 33342 was used to observe morphological changes during apoptosis. Moreover, quantitative real time PCR and western blot analysis were used to detect the effect of cinnamaldehyde on human gastric carcinoma cell line BGC-823. The results showed that compared with the control group, einnamaldehyde had inhibitory effect on human gastric carcinoma cell line BGC-823 （P 〈 0.01）. It showed that cinnamaldehyde induced apoptosis through the downregulation of Bcl-2, Bcl-xL and Survivin expression, upregulation of Bax and Bak expression, downregulation of Bcl-2 and Procaspase-3, and upregulation of BAX. It was concluded that cinnamaldehyde had inhibitory effect on the proliferation of human gastric carcinoma cell line BGC-823 and induced apoptosis. It may be related to the activation of the endogenous apoptosis pathway.

关 键 词：桂皮醛 人胃癌BGC-823细胞 细胞增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]