超高效液相色谱-串联质谱法测定食品中烯肟菌酯残留量  

UHPLC-MS/MS Determination of Residual Amount of Enostrobilurin in Food

作　　者：吴洁珊[1] 陈亮[1] 冯家望[1] 任永霞[1] 蔡勤仁[1] 

机构地区：[1]珠海出入境检验检疫局,珠海519015

出　　处：《理化检验（化学分册）》2013年第8期953-956,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家认监委项目(2010B287K)

摘　　要：提出了用超高效液相色谱一串联质谱法测定食品中烯肟菌酯残留量的方法。样品经乙腈提取后，以伯仲胺、c18和无水硫酸镁为萃取剂用基质分散固相萃取净化，净化液经EclipsePlusC，8色谱柱（50mmX2．1mm，1．8μm）分离，用乙腈与5mmol-L-1。乙酸铵溶液（75＋25）的混合液洗脱，采用正离子模式监测。烯肟菌酯的质量浓度在50μg·L-1以内与峰面积呈线性关系，检出限（3S／N）为1肚g·kg-1，以空白样品为基体进行加标回收试验，所得回收率在76．7％～89．6％之间，相对标准偏差（n=10）在4．2％～11％之间。UHPLC-MS/MS was applied to determination of residual amount of enostrobilurin in food . The sample was extracted with acetonitrile and purified by dispersive solid phase extraction with primary secondary amine, Cla and anhydrous MgSO4 as extractants. The eluate obtained were used for UHPLC-MS/MS determination, in which Eclipse Plus C18 column （50 mm）〈 2. 1 mm, 1. 8 μm）was used as stationary phase, and a mixture of acetonitrile and 5 mmol L-1 ammonium acetate （75 ＋25） was used as mobile phase; positive electrospray ionization was used in the detection. Linear relationship between values of peak area and mass concentration of enostrobilurin was kept in the range within 50 g L , with detection limit （3S/N） of 1 μg kg 1. On the base of blank sample, test for recovery was made by standard addition method; values of average recovery found were in the range of 76.7--89.6%, with RSD＇s （n=10） in the range of 4.2%--11%

关 键 词：超高效液相色谱-串联质谱法 烯肟菌酯 食品 

分 类 号：O657.63[理学—分析化学]