急性染镉对大鼠心肌细胞闰盘超微结构和连接蛋白43表达的影响及黄芪甲苷的拮抗作用  

Astragaloside Ⅳ antagonizes effects of cadmium on cardiomyocyte intercalated disc ultrastructure and expression of connexin 43 in rat

作　　者：姚志勇[1] 廖晓岗[1] 宁巍[1] 王红[1] 范京川[1] 李兆飞[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016

出　　处：《解剖学杂志》2013年第4期732-735,767,共5页Chinese Journal of Anatomy

摘　　要：目的：观察急性染镉对大鼠心肌细胞闰盘超微结构和连接蛋白43（Cx43）表达的影响,探讨黄芪甲苷的保护机制.方法：SD大鼠随机分为正常组、镉处理组、镉加黄芪甲苷处理组,取心室肌组织,分别作光镜、透射电镜观察和免疫组织化学检测.结果：与正常组相比,镉组心肌细胞连接蛋白43的表达量明显降低,心肌纤维和闰盘超微结构破坏严重；而镉加黄芪甲苷组心肌细胞连接蛋白43的表达量较镉组显著增加,心肌纤维和闰盘超微结构的损伤也明显减轻.结论：镉能破坏心肌细胞闰盘超微结构,影响连接蛋白43的表达及分布,黄芪甲苷能在一定程度上拮抗镉对心肌细胞闰盘超微结构和连接蛋白43的毒性损伤.Objective： To observe the effect of cadmium （Cd） op cardiomyocyte intercalated disc ultrastructure and expression of connexin 43 （Cx43） in rats, and the protection mechanism of astragaloside 1V （Aslg） on it. Methods： SD rats were randomly divided into 3 groups： control group, CA group （rats were treated with Cd 1 mg· d-1 ·kg-1 body weight by intraperitoneal injection）, CA＋As IV group （the dose of cadmium was 1 mg· d-1· kg-1 body weight, the dose of As IV respectively were 5 mg · d-1· kg_1 body weight and 10 mg·d · kg-1 body weight）. The rats after 1-week or 2-week treatment were anesthetized for thoracotomy, the ventricular tissues were respectively adopted for light and electron microscope observation and immunohistochemistry. Results： The expression of Cx43 was significantly reduced, and cardiac muscle fibers and intercalated disc ultrastructure were both damaged in CA group; however, those in CA＋ As IV were improved when compared with CA group. Conclusion： Cd can damage cardiomyocyte intercalated disc ultrastructure, and reduce expression of Cx43~ astragaloside IV retains the expression level of Cx43 and maintains morphologic integrity of cardiomyoeyte intercalated disc.

关 键 词：黄芪甲苷 镉 心肌细胞 连接蛋白43 超微结构 

分 类 号：R737.25[医药卫生—肿瘤]