补阳还五汤联合神经干细胞移植促进脊髓损伤髓鞘再生  

Effect of Buyang-Huanwu decoction combined with neural stem cells on remyelination after spinal cord injury in rat

作　　者：柴勇[1] 张敏[1] 刘文波[2] 张瑾锦[2] 张立霞[2] 杨成[1] 

机构地区：[1]滨州医学院,人体解剖学教研室,烟台264003 [2]滨州医学院,医学研究中心,烟台264003

出　　处：《解剖学杂志》2013年第4期785-788,共4页Chinese Journal of Anatomy

基　　金：山东省优秀中青年科学家奖励基金（2004BS02017）;山东省自然科学基金（Y2007C034）

摘　　要：目的：探讨补阳还五汤（BYHWD）灌胃联合神经干细胞（NSCs）移植对脊髓损伤（SCI）后髓鞘再生的影响.方法：将48只成年雌性SD大鼠随机分为脊髓损伤（SCI）组、BYHWD灌胃组、NSCs移植组、NSCs移植联合BYHWD灌胃（BYHWD＋ NSCs）组.SCI组不做治疗；NSCs组于脊髓横断后局部移植NSCs; BYHWD＋ NSCs组移植NSCs,并每天BYHWD灌胃；各组在损伤后14、28 d后分别留取损伤脊髓标本,用免疫荧光双标法检测髓磷脂碱性蛋白（MBP）表达情况,透射电镜观察脊髓白质内髓鞘超微结构变化.结果：BYHWD＋ NSCs组MBP阳性表达最多,较其他3组差异有统计学意义.电镜下BYHWD＋NSCs组髓鞘结构较为规整,轴突微丝、微管排列有序,线粒体结构完整,可见薄髓的再生髓鞘.结论：SCI后BYHWD灌胃联合NSCs移植能够促进损伤脊髓MBP的表达及脱髓的轴突再髓鞘化.Objective： To investigate the effect of Buyang-Huanwu Decoction（BYHWD） combined with neural stem cells （NSCs） on remyelination of the injured spinal cord in rats. Methods.. 48 adult female SD rats were divided into four groups randomly： spinal cord injury （SCI） group, NSCs transplantation group, BYHWD administration group and NSCs transplantation combined with BYI-I.WD administration（BYHWDTNSCs） group. There was no treatment in the SCI group. NSCs were transplanted into the injured site after the spinal cord was transected in the NSCs group. BYHWD was administrated every day after NSCs were transplanted in the BYHWD＋NSCs group. The samples of the injured spinal cords in each group were obtained at 14 d and 28 d after the models were established. The myelin basic protein （MBP） was examined by double immunofluorescence staining technique. The ultrastructure of the myelin sheath in the spinal cord white matter was observed under a transmission electron microscope（TEM）. Results： The expression of MBP in the BYHWD＋ NSCs group was more obvious than that in other groups. TEM showed： In the BYHWD＋NSCs group, the structure of myelin sheaths was clear and neat, the microfilaments and microtubules arranged orderly, the structure of mitochondria was intact, and the thin regenerated myelin sheath could be detected frequently. Conclusion： BYHWD administration combined with NSCs transplantation can promote the expression of MBP and remyelination of the injured spinal cord in rats.

关 键 词：脊髓损伤 神经干细胞 补阳还五汤 髓鞘再生 大鼠 

分 类 号：R651.2[医药卫生—外科学]