汉防己甲素对胃癌BGC-823细胞增殖和凋亡的影响  

Proliferation inhibition and apoptosis induction of Tetrandrine in gastric cancer BGC- 823 cells

作　　者：秦蓉[1] 曹渊[1] 方悦[1] 谢婵娟[1] 陈巧云[1] 沈慧玲[1] 许文林[1] 

机构地区：[1]江苏大学附属人民医院肿瘤科,江苏镇江212000

出　　处：《现代肿瘤医学》2013年第9期1911-1914,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(NO.81070423;NO.81101677);江苏省自然科学基金资助项目(NO.BK2010332)

摘　　要：目的:探讨汉防己甲素对人胃癌BGC-823细胞株的增殖抑制与诱导凋亡的作用。方法:通过MTT实验检测汉防己甲素对于细胞生长的抑制率,流式细胞术检测细胞凋亡比,Western blotting检测凋亡相关蛋白表达。结果:汉防己甲素显著抑制BGC-823细胞生长,呈时间-剂量依赖性。流式细胞术检测显示不同浓度汉防己甲素干预后,凋亡细胞比例明显增高,且呈浓度-时间依赖性。Western blotting检测显示Bax,Bad和Bak蛋白表达增加,Bcl-2和Bcl-xl蛋白表达降低,Caspase-3和Caspase-9表达增加,用Caspase抑制剂z-VAD-fmk预处理4h后,z-VAD-fmk能抑制汉防己甲素引起的Caspase-3表达增加。结论:在一定条件下,汉防己甲素能够抑制胃癌BGC-823细胞增殖并且诱导其发生凋亡。Objective：To study the effects of Tetrandrine on proliferation-inhibited and apotosis-induced effect of gastric cancer BGC-823 cells.Methods：The growth of BGC-823 cells treated with Tetrandrine was investigated by MTT proliferation assays.Cell apoptosis was quantified with flow cytometry using Annexin-V-PI staining.The expression of apoptosis-related protein was analyzed by Western blotting.Results：Tetrandrine treatment showed strong growth inhibitory and apoptotic effects on human gastric cancer BGC-823 cells in a time-and concentration -dependent manner.Western blotting showed the up-regulation of Bax,Bak and Bad,down-regulation of Bcl-2 and Bcl-xl,the activation of Caspase-3 and-9.Meanwhile,pretreatment with the pan-Caspase inhibitor z-VAD-fmk reduced Tetrandrine-induced apoptosis by blocking activation of Caspases.Conclusion：In certain conditions,Tetrandrine could inhibit proliferation and induce apoptosis in human gastric cancer BGC-823 cells.

关 键 词：汉防己甲素 BGC-823 增殖 凋亡 

分 类 号：R73-361[医药卫生—肿瘤] R735.2[医药卫生—临床医学]