白桦酯醇对人卵巢癌裸鼠移植瘤的抑制作用  

Effect of Betulin in inhibiting the growth of human ovarian cancer nude mice tumor

作　　者：刘佳[1] 王丹云[1] 李雪婷[1] 张淑敏[1] 邵婷[1] 张宗臻[1] 刘运铎[1] 陈秀玮[1] 

机构地区：[1]哈尔滨医科大学附属第三医院妇科,黑龙江哈尔滨150081

出　　处：《现代肿瘤医学》2013年第9期1933-1937,共5页Journal of Modern Oncology

基　　金：黑龙江省哈尔滨市科技创新人才研究专项资金项目(编号:2012RFLXS015)

摘　　要：目的:观察白桦酯醇对人卵巢癌裸鼠移植瘤的抑制作用及对Bcl-2和Caspase-3表达的影响。方法:建立卵巢癌SKOV3裸鼠移植瘤模型,将30只荷瘤鼠随机分为5组:空白对照组、白桦酯醇高、中、低浓度组和顺铂组(阳性对照)。21天后处死裸鼠,称取瘤重量,观察抑瘤率;免疫组织化学SP法检测移植瘤Bcl-2和Caspase-3的表达。结果:高、中、低组抑瘤率为54.19%、35.75%、20.67%,与空白对照组比较差异有统计学意义(P<0.05);高剂量组抑瘤率与顺铂组无差异(P>0.05)。免疫组化显示,各治疗组Bcl-2的表达较空白对照组明显下调;Caspase-3的表达较空白组明显活化,其高剂量组表达与阳性药物顺铂相当。结论:白桦酯醇有一定的抑制卵巢癌生长的作用,可能与其诱导细胞凋亡有关。Objective：To observe the effect of Betulin in inhibiting the growth of human ovarian cancer nude mice tumor and the expression of Bcl-2 and Caspase-3.Methods：Nude mice model with transplanted tumor of human orarian cancer SKOV3 cells were established successfully.Thirty nude mice with transplanted SKOV3 tumor were randomly divided into five groups：control group,Betulin high dosage group,Betulin medium dosage group,Betulin low dosage group and DDP group.At day 21 after treatment,the nude mice were executed,then the tumor was weighed.The inhibitive rates of the tumor in nude mice were observed and immunohistochemistry with SP staining method were used to detect the expression of Bcl-2 and Caspase-3.Results：The inhibitive rates of the tumor in Betulin treated group was 54.19％,35.75％,20.67％,the average tumor weight had significant difference with that in control group （P ＜ 0.05）.The Betulin high dosage group had no difference with that in DDP group （P ＞ 0.05） ; The Bcl-2 expression determined by immunohistochemical staining was down-regulated after treatment and compared with the control group,expressions of Caspase-3 in transplanted tumors were up-regulated in Betulin groups.The high dosage group expression had no significant difference with that in DDP group.Conclusion：Betulin has good effect in inhibiting the growth of ovarian cancer,which might be mediated by apoptosis.

关 键 词：白桦酯醇 裸鼠 卵巢癌 BCL-2 CASPASE-3 凋亡 

分 类 号：R73-361[医药卫生—肿瘤] R737.31[医药卫生—临床医学]