胃癌患者血清VEGF、COX-2表达的检测及临床意义  

Seurm levels of VEGF and COX - 2 in gastric cancer

作　　者：周海存[1] 刘宏斌[2] 苏琳[2] 李洪涛[2] 韩晓鹏[2] 朱万坤[2] 曹廷宝[2] 

机构地区：[1]兰州大学第二医院普外科,甘肃兰州730050 [2]兰州军区总医院普外科,甘肃兰州730050

出　　处：《现代肿瘤医学》2013年第9期2019-2021,共3页Journal of Modern Oncology

基　　金：甘肃省科技厅科技重大专项资助项目(编号:2010GS04390)

摘　　要：目的:检测胃癌患者血清中血管内皮生长因子(VEGF)、环氧合酶2(COX-2)的表达水平,探讨二者相关性及临床意义。方法:采用酶联免疫吸附试验(ELISA法)定量检测60例胃癌患者和30例健康人血清VEGF和COX-2水平。比较胃癌患者与健康人血清VEGF、COX-2浓度的差异。同时比较不同分期肿瘤患者血清VEGF、COX-2浓度的差异,并进行二者相关性分析。结果:胃癌患者血清VEGF水平(424.07±81.85pg/ml)明显高于健康人(117.23±20.09pg/ml)(P<0.01)。其水平与肿瘤大小、浸润深度、分化程度、有无转移、TNM分期有关(P<0.05)。血清COX-2水平(41.43±9.52ng/ml)也明显高于健康人(16.88±6.27ng/ml)(P<0.05)。其含量与胃癌分化程度、有无转移、TNM分期有关(P<0.05)。二者的表达呈显著正相关(r=0.618,P<0.01)。结论:胃癌患者血清中VEGF和COX-2高表达,其表达水平与肿瘤的分期相关,提示VEGF和COX-2在胃癌的发生和发展中发挥重要的作用。二者密切相关,在胃癌的诊断、治疗和预后评价中具有一定价值。Objective：To investigate the expression of the seurm levels of VEGF and COX-2 in gastric cancer and the clinical significance.Methods：Enzyme-linked immunosorbent assay（ELISA） was used to detect the serum level of VEGF and COX-2 in 60 gastric cancer patients and 30 healthy controls,comparing the serum level of VEGF and COX-2 between the two groups,and comparing the expression of the serum VEGF and COX-2 in different TNM stage,lymph node metastasis and distal metastasis in gastric cancer.Results：The expression levels of serum VEGF and COX-2 in gastric carcinoma were higher than those of the healthy controls（P ＜ 0.01）.The expression levels of serum VEGF and COX-2 was related to TNM stage,depth of invasion,differentiation,lymph node metastasis and distant metastasis（P ＜0.05）.The expression levels of serum VEGF was correlated with serum COX-2 expression （P ＜ 0.05）.Conclusion：VEGF and COX-2 are over expressed in gastric cancer,and increased of serum VEGF and COX-2 expression in gastric cancer were associated with maligment behavior.VEGF expression in gastric cancer was associated with COX-2 expression.Serum VEGF and COX-2 could be used as markers for the diagnosis,therapy and prognosis of gastric cancer.

关 键 词：胃癌 VEGF COX-2 

分 类 号：R735.2[医药卫生—肿瘤]