miR-455在宫颈癌中的表达及其与MVD、VEGF表达的相关性研究  

Expression of miR- 455 and its relationship with MVD,VEGF in cervical cancer

作　　者：李平[1] 宋鉴清[2] 王学梅[1] 张雨芹[1] 刘艳君[1] 陶春梅[1] 

机构地区：[1]中国医科大学附属第一医院超声诊断科,辽宁沈阳110001 [2]中国医科大学附属第一医院检验科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2013年第9期2058-2061,共4页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81171650);辽宁省自然科学基金资助项目(编号:201102291)

摘　　要：目的:探讨miR-455在宫颈癌中的表达及其与微血管密度(MVD)及血管内皮生长因子(VEGF)表达的关系。方法:采用实时荧光定量PCR技术检测68例宫颈癌患者和49例慢性宫颈炎(对照组)组织中miR-455的表达。免疫组化方法检测组织内MVD及VEGF蛋白表达水平。结果:宫颈癌组织内miR-455的相对表达量显著低于慢性宫颈炎(1.17±0.59 vs 2.58±0.83,P<0.01)。68例宫颈癌标本MVD表达的计数结果为30.45±7.9。Pearson相关分析结果显示,宫颈癌组织内miR-455表达水平与MVD呈负相关(r=-0.835,P<0.01。宫颈癌VEGF表达为阴性、弱阳性、中度阳性和强阳性组中,miR-455相对表达定量(RQ)分别为1.77±0.58、1.59±0.50、1.34±0.35和0.68±0.26,Spearman等级相关分析结果显示,miR-455表达水平与VEGF的表达呈负等级相关(r s=-0.857,P<0.01)。结论:miR-455低表达可能通过VEGF信号通路促进宫颈癌内微血管形成,是一个潜在的抑癌miRNA。Objective：To investigate the expression of miR-455 in cervical cancer and its relationship with microvascular density（MVD） and vascular endothelial growth factor （VEGF）.Methods：The expression of miR-455 in 68 cases with cervical cancer and 49 cases with chronic cervicitis（control group） was tested by real time PCR.The expression of MVD and VEGF were examined by immunohistochemistry.Results：The relative quantity of miR-455 expression in cervical cancer tissue was significantly lower than that in chronic cervicitis （1.17 ±0.59 vs 2.58 ±0.83,P ＜ 0.01）.The result of MVD was 30.45 ± 7.9 in 68 cases of cervical cancer,and the expression of miR-455 was negatively correlated with MVD by Pearson correlation analysis （r =-0.835,P ＜ 0.01）.The expression of VEGF was negative,weakly positive,moderately positive,and strongly positive in the specimen of cervical cancer,and the expression of miR-455 was 1.77 ± 0.58,1.59 ± 0.50,1.34 ± 0.35 and 0.68 ± 0.26 respectively.Spearman rank correlation analysis showed that the expression of miR-455 was negatively correlated with that of VEGF （rs =-0.857,P ＜ 0.01）.Conclusion：Low expression of miR-455 promotes the formation of microvascular in cervical cancer,and it may be a potential tumor suppressor gene.

关 键 词：宫颈癌 miR-455 微血管密度(MVD) 血管内皮生长因子(VEGF) 

分 类 号：R737.33[医药卫生—肿瘤]