多发性骨髓瘤患者外周血miR-21的表达与意义  

Expression and meaning of miR - 21 in multiple myeloma(MM) patients

作　　者：王金行[1] 周雯雯[1] 刘柏新[1] 满冬亮[1] 杨志东[1] 宋鉴清[1] 

机构地区：[1]中国医科大学附属第一医院检验科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2013年第9期2089-2091,共3页Journal of Modern Oncology

基　　金：辽宁省教育厅科研项目计划(编号:201102291)

摘　　要：目的:检测多发性骨髓瘤(multiple myeloma,MM)患者外周血miR-21表达水平,探讨miR-21在MM发生、发展中的调控作用及临床意义。方法:选择30例MM患者、15例MGUS患者及20例正常对照的,测定其血清β2-MG、IgA、IgM、IgG、λ、κ、TP、ALB、Hb、LDH及Ca2+等水平含量。用SYBR Green实时荧光定量PCR检测miR-21表达水平。相关检验分析MM患者外周血miR-21水平与临床指标相关性。结果:MM组血清循环miR-21的表达水平较MGUS组和NC组显著增高,差异有统计学意义(P<0.01)。根据ISS分期,MM组中Ⅰ期miR-21、IgG、κ及ALB水平较Ⅱ期和Ⅲ期明显降低或增高,Ⅲ期IgA、β2-MG和λ的水平较Ⅰ期和Ⅱ期显著增高,差异均有统计学意义(P<0.05或P<0.01)。MM患者miR-21水平与κ、(κ+λ)、IgG、(IgG+IgA+IgM)和β2-MG呈正相关,与ALB呈负相关(均P<0.01)。结论:miR-21过表达在MM的发病中发挥着重要的致癌基因作用,miR-21与MM的发生发展和疾病预后有密切关系,可作为判断MM患者预后不良指标之一。Objective：To study the expression level of peripheral mir-21 in the multiple myeloma（multiple myeloma,MM） patients and clinical significance.Methods：To choose 30 MM patients,15 MGUS patients and 20 normal control,and determine the level of serum β2-MG,IgA and IgM,IgG,λ,κ,TP,ALB,Hb,LDH and Ca2＋,test the expression level of mir-21 by SYBR green real-time fluorescent quantitative PCR detection.Results：The expression level of serum mir-21 in MM group was significantly higher than MGUS group and NC group,（P ＜0.01）.According to the ISS installment,the level of mir-21,lgG,κ and ALB in MM group in stage Ⅰ decreased or increased,comparing with stage Ⅱ and Ⅲ.The level of IgA,β2-MG and λ in stage Ⅲ was higher than stage Ⅰ and Ⅱ （P ＜0.05 or P ＜ 0.01）.The levels of MM patients mir-21 and κ,（κ ＋ λ）,IgG,（IgG ＋ IgA ＋ IgM） and β2-MG was positively correlated with ALB （P ＜ 0.01）.Conclusion：miR-21 plays an important role as oncogene,mir-21 is important in the occurrence,development and disease prognosis of MM.

关 键 词：多发性骨髓瘤 MIR-21 PCR 相关性 

分 类 号：R733.3[医药卫生—肿瘤]