CMTM8与E-cadherin在原发性和转移性肾透明细胞癌中的表达  

Expressions of CMTM8and E-cadherin in primary and metastatic clear cell renal cell carcinoma

作　　者：胡浩[1] 陈京文[1] 许克新[1] 王栋[1] 王应[2] 王功伟[3] 张石英[1] 王晓峰[1] 

机构地区：[1]北京大学人民医院泌尿外科,北京100044 [2]北京大学人类疾病基因研究中心,北京100191 [3]北京大学人民医院病理科,北京100044

出　　处：《北京大学学报（医学版）》2013年第4期537-541,共5页Journal of Peking University:Health Sciences

摘　　要：目的:探讨CMTM8及E-cadherin在原发性和转移性肾透明细胞癌组织中的表达及其临床病理意义。方法:采用免疫组织化学方法检测17例原发性肾透明细胞癌组织、8例癌旁正常肾组织、9例肾透明细胞癌肺转移组织和10例肾透明细胞癌骨转移组织的CMTM8及E-cadherin表达。结果:CMTM8和E-cadherin在正常肾小管上皮细胞膜上呈强阳性表达;在低级别肾透明细胞癌的细胞膜上表达强度明显下降,但仍呈完整连续的细胞膜表达;在高级别的肾透明细胞癌中,其在细胞膜上的表达强度进一步减弱,表达不连续,同时开始出现细胞浆的表达。CMTM8在肾透明细胞癌肺转移及骨转移组织中的细胞膜表达水平进一步下降,以细胞浆表达为主。E-cadherin在转移癌组织中的表达可为阴性,也可为细胞膜和细胞浆的弱阳性。CMTM8和E-cadherin均与肾透明细胞癌的进展和转移有显著负相关(r值分别为-0.841和-0.732,P<0.001),两者间也有显著相关性(r值为0.694,P<0.001)。结论:CMTM8和E-cadherin与肾透明细胞癌的发生和进展存在明显的负相关性,两者在细胞中表达的部位和强度对判断肿瘤预后有重要意义。Objective：To investigate the expression and clinical pathological significance of CMTM8 and E-cadherin in primary and metastatic clear-cell renal cell carcinoma.Methods： The immunohistochemistry was used to detect the expressions of CMTM8 and E-cadherin in 17 cases of primary clear-cell renal cell carcinoma,8 cases of normal renal tissue,9 cases of metastatic renal cell carcinoma in lungs and 10 cases of metastatic renal cell carcinoma in bones.Results： The membranous staining intensities of CMTM8 and E-cadherin in normal renal tissue were strong,but reduced in low-grade clear-cell renal cell carcinoma.There were positive cytoplasmic stainings of CMTM8 and E-cadherin in high-grade clear-cell renal cell carcinoma.Increased cytoplasmic expression of CMTM8 was frequent in metastatic renal cell carcinoma,accompanied with reduced cell surface staining.The expression of E-cadherin could be negative or weakly positive at membrane and cytoplasma.CMTM8 and E-cadherin expressions were negatively correlated with development and metastasis in clear-cell renal cell carcinoma（r=-0.841 and r=-0.732,P＆lt;0.001）.Moreover,CMTM8 was also correlated with the expression of E-cadherin（r=0.694,P＆lt;0.001）.Conclusion： CMTM8 and E-cadherin are negatively correlated with tumorgenesis and development in clear-cell renal cell carcinoma.The location and intensity of their expressions have significant association with the prognosis.

关 键 词：癌 肾细胞 腺癌 透明细胞 钙黏着糖蛋白类 CMTM8 

分 类 号：R737.11[医药卫生—肿瘤]